Removal of divalent cations induces structural transitions in Red clover necrotic mosaic virus, revealing a potential mechanism for RNA release

Michael Sherman, Richard H. Guenther, Florence Tama, Tim L. Sit, Charles L. Brooks, Albert M. Mikhailov, Elena V. Orlova, Timothy S. Baker, Steven A. Lommel

Research output: Contribution to journalArticle

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Abstract

The structure of Red clover necrotic mosaic virus (RCNMV), an icosahedral plant virus, was resolved to 8.5 Å by cryoelectron microscopy. The virion capsid has prominent surface protrusions and subunits with a clearly defined shell and protruding domains. The structures of both the individual capsid protein (CP) subunits and the entire virion capsid are consistent with other species in the Tombusviridae family. Within the RCNMV capsid, there is a clearly defined inner cage formed by complexes of genomic RNA and the amino termini of CP subunits. An RCNMV virion has approximately 390 ± 30 Ca2+ ions bound to the capsid and 420 ± 25 Mg2+ ions thought to be in the interior of the capsid. Depletion of both Ca2+ and Mg 2+ ions from RCNMV leads to significant structural changes, including (i) formation of 11- to 13-Å-diameter channels that extend through the capsid and (ii) significant reorganization within the interior of the capsid. Genomic RNA within native capsids containing both Ca2+ and Mg 2+ ions is extremely resistant to nucleases, but depletion of both of these cations results in nuclease sensitivity, as measured by a significant reduction in RCNMV infectivity. These results indicate that divalent cations play a central role in capsid dynamics and suggest a mechanism for the release of viral RNA in low-divalent-cation environments such as those found within the cytoplasm of a cell.

Original languageEnglish (US)
Pages (from-to)10395-10406
Number of pages12
JournalJournal of Virology
Volume80
Issue number21
DOIs
StatePublished - Nov 2006

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Red clover necrotic mosaic virus
Tombusviridae
capsid
Capsid
Divalent Cations
cations
RNA
virion
Virion
Ions
ions
nucleases
Protein Subunits
Capsid Proteins
protein subunits
coat proteins
calcium
Cryoelectron Microscopy
genomics
Plant Viruses

ASJC Scopus subject areas

  • Immunology

Cite this

Removal of divalent cations induces structural transitions in Red clover necrotic mosaic virus, revealing a potential mechanism for RNA release. / Sherman, Michael; Guenther, Richard H.; Tama, Florence; Sit, Tim L.; Brooks, Charles L.; Mikhailov, Albert M.; Orlova, Elena V.; Baker, Timothy S.; Lommel, Steven A.

In: Journal of Virology, Vol. 80, No. 21, 11.2006, p. 10395-10406.

Research output: Contribution to journalArticle

Sherman, M, Guenther, RH, Tama, F, Sit, TL, Brooks, CL, Mikhailov, AM, Orlova, EV, Baker, TS & Lommel, SA 2006, 'Removal of divalent cations induces structural transitions in Red clover necrotic mosaic virus, revealing a potential mechanism for RNA release', Journal of Virology, vol. 80, no. 21, pp. 10395-10406. https://doi.org/10.1128/JVI.01137-06
Sherman, Michael ; Guenther, Richard H. ; Tama, Florence ; Sit, Tim L. ; Brooks, Charles L. ; Mikhailov, Albert M. ; Orlova, Elena V. ; Baker, Timothy S. ; Lommel, Steven A. / Removal of divalent cations induces structural transitions in Red clover necrotic mosaic virus, revealing a potential mechanism for RNA release. In: Journal of Virology. 2006 ; Vol. 80, No. 21. pp. 10395-10406.
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