Renaturation of DNA catalysed by yeast DNA repair and recombination protein RAD10

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

THE RAD10 gene of Saccharomyces cerevisiae is required for the incision step of excision repair of ultraviolet-damaged DNA (refs 1, 2), and it functions in mitotic recombination3. RAD10 has homology to the human excision repair gene ERCC-1 (ref. 4). Here we describe the purification of the protein encoded by RAD10 and show that it is a DNA-binding protein with a strong preference for single-stranded DNA. We also show that RAD10 promotes the renaturation of complementary DNA strands.

Original languageEnglish (US)
Pages (from-to)743-745
Number of pages3
JournalNature
Volume355
Issue number6362
StatePublished - Feb 20 1992
Externally publishedYes

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DNA Repair
Genetic Recombination
Yeasts
Single-Stranded DNA
DNA
DNA-Binding Proteins
Genes
Saccharomyces cerevisiae
Proteins
Complementary DNA

ASJC Scopus subject areas

  • General

Cite this

Renaturation of DNA catalysed by yeast DNA repair and recombination protein RAD10. / Sung, Patrick; Prakash, Louise; Prakash, Satya.

In: Nature, Vol. 355, No. 6362, 20.02.1992, p. 743-745.

Research output: Contribution to journalArticle

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