The ability to remove ultraviolet (UV)-induced pyrimidine dimers was examined in four radiation-sensitive mutants of Saccharomyces cerevisiae. The susceptibility of DNA from irradiated cells to nicking by either the T4 UV-endonuclease or an endonuclease activity found in crude extracts of Micrococcus luteus was used to measure the presence of dimers in DNA. The rad3 and rad4 mutants are shown to be defective in dimer excision whereas the rad6 and rad9 mutants are proficient in dimer excision.
|Number of pages
|Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
|Published - Oct 1977
ASJC Scopus subject areas
- Molecular Biology
- Health, Toxicology and Mutagenesis