Repeatability of corneal densitometry measurements using a scheimpflug camera in healthy normal corneas

Mojgan Pakbin, Mehdi Khabazkhoob, Mohammad Pakravan, Akbar Fotouhi, Ebrahim Jafarzadehpur, Mohamadreza Aghamirsalim, Hassan Hashemi

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


Purpose: To determine the repeatability of corneal densitometry measured by the Scheimpflug imaging system. Methods: This cross-sectional study was conducted on photorefractive keratectomy candidates. One eye of each participant underwent imaging using Pentacam HR three times, 10 min apart. The repeatability of densitometry measurements was evaluated in four concentric annuli around the corneal apex and in different corneal depths. The repeatability of the measurements was evaluated using the intraclass correlation coefficient (ICC), repeatability coefficient (RC), and coefficient of variation (CV). The difference of repeatability between layers and zones was tested by tolerance index (TI). Results: Sixty eyes of sixty patients with a mean age of 27.76 ± 3.93 years were studied. Half of the participants were female (n = 30, 50%). ICC was above 0.9 in all corneal parts. The posterior layer and central zones showed the least variability of densitometry measurements considering the CV values. The RC was 2.06, 1.17, and 0.92 in anterior, central, and posterior layers, respectively. The RC was 0.88, 0.71, 1.51, and 4.56 in 0-2, 2-6, 6-10, and 10-12 mm circles, respectively. Only the reliability of densitometry in 10-12 mm annulus was statistically lower than the central zone (TI = 0.71). Conclusions: Corneal densitometry measurements provided by the Pentacam had good repeatability. The repeatability of densitometry measurements decreased from the center to the periphery (with an exception for 0-2 mm and 2-6 mm) and from the posterior to the anterior of the cornea. The reliability of the 10-12 mm zone was markedly less than other zones.

Original languageEnglish (US)
Pages (from-to)50-55
Number of pages6
JournalJournal of Current Ophthalmology
Issue number1
StatePublished - Jan 1 2022
Externally publishedYes


  • Corneal densitometry
  • Reliability
  • Scheimpflug imaging system

ASJC Scopus subject areas

  • Ophthalmology


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