Repeated immunolesions display diminished stress response signal

Zezong Gu, Juan Yu, Karin Werrbach-Perez, J. Regino Perez-Polo

    Research output: Contribution to journalArticle

    1 Citation (Scopus)

    Abstract

    Cholinergic basal forebrain neurons (CBFNs) retrogradely transport neurotrophins released in the hippocampus and cortex as part of a general response to injury in a process that is impaired in the aged rodent and can be spared by the exogenous addition of pharmacological doses of nerve growth factor (NGF). This observation suggests that components of stress response signal transduction pathways in the aged CNS can be exogenously activated. The extent and mechanism of the endogenous stimulation of NGF in response to injury can be mimicked via treatment with 192 IgG-saporin of rat CNS, an immunolesion model. Here we report on the use of a conditioning lesion paradigm to determine if repeated partial immunolesions have a conditioning effect on the immunolesion-induced increases in NGF protein or decreases in choline acetyltransferase (ChAT) and acetylcholinesterase (AChE) activity.We report that chronic repeated immunolesions, as used here, were not as effective as a one time equivalent immunolesion in terms of induced NGF protein increases or decreasing ChAT and AChE activity in the hippocampus and cortex. Thus, chronic lesions resulting in cholinergic impairment typical of the aged CNS may differ from acute toxic models as a result of desensitization due to a conditioning effect of chronic subthreshold lesioning events in the CNS. Copyright (C) 2000 ISDN.

    Original languageEnglish (US)
    Pages (from-to)177-183
    Number of pages7
    JournalInternational Journal of Developmental Neuroscience
    Volume18
    Issue number2-3
    DOIs
    StatePublished - Jun 2000

    Fingerprint

    Nerve Growth Factor
    Choline O-Acetyltransferase
    Acetylcholinesterase
    Cholinergic Agents
    Hippocampus
    Poisons
    Nerve Growth Factors
    Wounds and Injuries
    Rodentia
    Signal Transduction
    Proteins
    Pharmacology
    Neurons

    Keywords

    • 192 IgG-saporin
    • Acetylcholinesterase
    • Choline acetyltransferase
    • Cholinergic basal forebrain neuron
    • Conditioning
    • Immunolesion
    • Nerve growth factor

    ASJC Scopus subject areas

    • Developmental Biology
    • Developmental Neuroscience

    Cite this

    Repeated immunolesions display diminished stress response signal. / Gu, Zezong; Yu, Juan; Werrbach-Perez, Karin; Perez-Polo, J. Regino.

    In: International Journal of Developmental Neuroscience, Vol. 18, No. 2-3, 06.2000, p. 177-183.

    Research output: Contribution to journalArticle

    Gu, Zezong ; Yu, Juan ; Werrbach-Perez, Karin ; Perez-Polo, J. Regino. / Repeated immunolesions display diminished stress response signal. In: International Journal of Developmental Neuroscience. 2000 ; Vol. 18, No. 2-3. pp. 177-183.
    @article{d4596426b10a41189754543a68103707,
    title = "Repeated immunolesions display diminished stress response signal",
    abstract = "Cholinergic basal forebrain neurons (CBFNs) retrogradely transport neurotrophins released in the hippocampus and cortex as part of a general response to injury in a process that is impaired in the aged rodent and can be spared by the exogenous addition of pharmacological doses of nerve growth factor (NGF). This observation suggests that components of stress response signal transduction pathways in the aged CNS can be exogenously activated. The extent and mechanism of the endogenous stimulation of NGF in response to injury can be mimicked via treatment with 192 IgG-saporin of rat CNS, an immunolesion model. Here we report on the use of a conditioning lesion paradigm to determine if repeated partial immunolesions have a conditioning effect on the immunolesion-induced increases in NGF protein or decreases in choline acetyltransferase (ChAT) and acetylcholinesterase (AChE) activity.We report that chronic repeated immunolesions, as used here, were not as effective as a one time equivalent immunolesion in terms of induced NGF protein increases or decreasing ChAT and AChE activity in the hippocampus and cortex. Thus, chronic lesions resulting in cholinergic impairment typical of the aged CNS may differ from acute toxic models as a result of desensitization due to a conditioning effect of chronic subthreshold lesioning events in the CNS. Copyright (C) 2000 ISDN.",
    keywords = "192 IgG-saporin, Acetylcholinesterase, Choline acetyltransferase, Cholinergic basal forebrain neuron, Conditioning, Immunolesion, Nerve growth factor",
    author = "Zezong Gu and Juan Yu and Karin Werrbach-Perez and Perez-Polo, {J. Regino}",
    year = "2000",
    month = "6",
    doi = "10.1016/S0736-5748(99)00086-6",
    language = "English (US)",
    volume = "18",
    pages = "177--183",
    journal = "International Journal of Developmental Neuroscience",
    issn = "0736-5748",
    publisher = "Elsevier Limited",
    number = "2-3",

    }

    TY - JOUR

    T1 - Repeated immunolesions display diminished stress response signal

    AU - Gu, Zezong

    AU - Yu, Juan

    AU - Werrbach-Perez, Karin

    AU - Perez-Polo, J. Regino

    PY - 2000/6

    Y1 - 2000/6

    N2 - Cholinergic basal forebrain neurons (CBFNs) retrogradely transport neurotrophins released in the hippocampus and cortex as part of a general response to injury in a process that is impaired in the aged rodent and can be spared by the exogenous addition of pharmacological doses of nerve growth factor (NGF). This observation suggests that components of stress response signal transduction pathways in the aged CNS can be exogenously activated. The extent and mechanism of the endogenous stimulation of NGF in response to injury can be mimicked via treatment with 192 IgG-saporin of rat CNS, an immunolesion model. Here we report on the use of a conditioning lesion paradigm to determine if repeated partial immunolesions have a conditioning effect on the immunolesion-induced increases in NGF protein or decreases in choline acetyltransferase (ChAT) and acetylcholinesterase (AChE) activity.We report that chronic repeated immunolesions, as used here, were not as effective as a one time equivalent immunolesion in terms of induced NGF protein increases or decreasing ChAT and AChE activity in the hippocampus and cortex. Thus, chronic lesions resulting in cholinergic impairment typical of the aged CNS may differ from acute toxic models as a result of desensitization due to a conditioning effect of chronic subthreshold lesioning events in the CNS. Copyright (C) 2000 ISDN.

    AB - Cholinergic basal forebrain neurons (CBFNs) retrogradely transport neurotrophins released in the hippocampus and cortex as part of a general response to injury in a process that is impaired in the aged rodent and can be spared by the exogenous addition of pharmacological doses of nerve growth factor (NGF). This observation suggests that components of stress response signal transduction pathways in the aged CNS can be exogenously activated. The extent and mechanism of the endogenous stimulation of NGF in response to injury can be mimicked via treatment with 192 IgG-saporin of rat CNS, an immunolesion model. Here we report on the use of a conditioning lesion paradigm to determine if repeated partial immunolesions have a conditioning effect on the immunolesion-induced increases in NGF protein or decreases in choline acetyltransferase (ChAT) and acetylcholinesterase (AChE) activity.We report that chronic repeated immunolesions, as used here, were not as effective as a one time equivalent immunolesion in terms of induced NGF protein increases or decreasing ChAT and AChE activity in the hippocampus and cortex. Thus, chronic lesions resulting in cholinergic impairment typical of the aged CNS may differ from acute toxic models as a result of desensitization due to a conditioning effect of chronic subthreshold lesioning events in the CNS. Copyright (C) 2000 ISDN.

    KW - 192 IgG-saporin

    KW - Acetylcholinesterase

    KW - Choline acetyltransferase

    KW - Cholinergic basal forebrain neuron

    KW - Conditioning

    KW - Immunolesion

    KW - Nerve growth factor

    UR - http://www.scopus.com/inward/record.url?scp=0034010399&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=0034010399&partnerID=8YFLogxK

    U2 - 10.1016/S0736-5748(99)00086-6

    DO - 10.1016/S0736-5748(99)00086-6

    M3 - Article

    C2 - 10715572

    AN - SCOPUS:0034010399

    VL - 18

    SP - 177

    EP - 183

    JO - International Journal of Developmental Neuroscience

    JF - International Journal of Developmental Neuroscience

    SN - 0736-5748

    IS - 2-3

    ER -