Reperfusion-Induced Neutrophil CD18 Polarization

Effect of Hyperbaric Oxygen

Kayvan T. Khiabani, Seth Bellister, Sarah S. Skaggs, Linda L. Stephenson, Chandra Nataraj, Wei Z. Wang, William A. Zamboni

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Background: Hyperbaric oxygen (HBO) inhibits ischemia reperfusion (IR) -induced neutrophil adhesion to endothelium through an unknown mechanism. This study evaluates the effect of HBO on IR-stimulated neutrophil adhesion and polarization of expressed CD18 adhesion molecules using a novel in vitro adhesion assay and confocal microscopy. Materials and methods: Neutrophils from normal animals were isolated from whole blood and incubated with plasma from rat gracilis muscle flaps on coverslips pretreated with ICAM. Percent adherence to ICAM and CD18 polarization was evaluated in the following five groups: (1) Nonischemic control, n = 15; (2) 4 h ischemia (IR, n = 15); (3) 4 h ischemia with HBO treatment (100% oxygen at 2.5 atmospheres absolute (IR + HBO, n = 15)); (4) 4 h ischemia with 100% oxygen at room temperature and pressure (RTP) (IR + normobaric hyperoxia, n = 5); and (5) 4 h ischemia with 8% oxygen at 2.5 atmospheres absolute (IR + hyperbaric normoxia, n = 5). Direct HBO treatment of neutrophils was also evaluated. Results: Neutrophils exposed to IR plasma showed a significant increase in percent adherent (0.8 ± 0.1% versus 16.7 ± 2.2%, P < 0.05) and polarized cells (6.2 ± 1.7% versus 43.9 ± 12.2%, P < 0.05) compared to controls. Hyperbaric oxygen significantly reduced the adhesion and polarization to 1.6 ± 0.3 and 4.1 ± 2.5%, respectively (P = < 0.05). Normobaric hyperoxia and hyperbaric normoxia did not affect neutrophil adherence or CD18 polarization following IR. Direct HBO treatment of neutrophils did not change the percent of polarized cells in IR. Conclusions: Hyperbaric oxygen inhibits IR-induced neutrophil adhesion by blocking CD18 surface polarization and requires plasma exposure to HBO. Treatment with oxygen or pressure alone is not effective.

Original languageEnglish (US)
Pages (from-to)11-16
Number of pages6
JournalJournal of Surgical Research
Volume150
Issue number1
DOIs
StatePublished - Nov 1 2008
Externally publishedYes

Fingerprint

Reperfusion
Neutrophils
Ischemia
Oxygen
Hyperoxia
Atmosphere
Pressure
Confocal Microscopy
Endothelium

Keywords

  • CD18 polarization
  • hyperbaric oxygen
  • ischemia reperfusion injury
  • polymorphonuclear leukocyte adhesion
  • skeletal muscle

ASJC Scopus subject areas

  • Surgery

Cite this

Khiabani, K. T., Bellister, S., Skaggs, S. S., Stephenson, L. L., Nataraj, C., Wang, W. Z., & Zamboni, W. A. (2008). Reperfusion-Induced Neutrophil CD18 Polarization: Effect of Hyperbaric Oxygen. Journal of Surgical Research, 150(1), 11-16. https://doi.org/10.1016/j.jss.2007.12.780

Reperfusion-Induced Neutrophil CD18 Polarization : Effect of Hyperbaric Oxygen. / Khiabani, Kayvan T.; Bellister, Seth; Skaggs, Sarah S.; Stephenson, Linda L.; Nataraj, Chandra; Wang, Wei Z.; Zamboni, William A.

In: Journal of Surgical Research, Vol. 150, No. 1, 01.11.2008, p. 11-16.

Research output: Contribution to journalArticle

Khiabani, KT, Bellister, S, Skaggs, SS, Stephenson, LL, Nataraj, C, Wang, WZ & Zamboni, WA 2008, 'Reperfusion-Induced Neutrophil CD18 Polarization: Effect of Hyperbaric Oxygen', Journal of Surgical Research, vol. 150, no. 1, pp. 11-16. https://doi.org/10.1016/j.jss.2007.12.780
Khiabani, Kayvan T. ; Bellister, Seth ; Skaggs, Sarah S. ; Stephenson, Linda L. ; Nataraj, Chandra ; Wang, Wei Z. ; Zamboni, William A. / Reperfusion-Induced Neutrophil CD18 Polarization : Effect of Hyperbaric Oxygen. In: Journal of Surgical Research. 2008 ; Vol. 150, No. 1. pp. 11-16.
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abstract = "Background: Hyperbaric oxygen (HBO) inhibits ischemia reperfusion (IR) -induced neutrophil adhesion to endothelium through an unknown mechanism. This study evaluates the effect of HBO on IR-stimulated neutrophil adhesion and polarization of expressed CD18 adhesion molecules using a novel in vitro adhesion assay and confocal microscopy. Materials and methods: Neutrophils from normal animals were isolated from whole blood and incubated with plasma from rat gracilis muscle flaps on coverslips pretreated with ICAM. Percent adherence to ICAM and CD18 polarization was evaluated in the following five groups: (1) Nonischemic control, n = 15; (2) 4 h ischemia (IR, n = 15); (3) 4 h ischemia with HBO treatment (100{\%} oxygen at 2.5 atmospheres absolute (IR + HBO, n = 15)); (4) 4 h ischemia with 100{\%} oxygen at room temperature and pressure (RTP) (IR + normobaric hyperoxia, n = 5); and (5) 4 h ischemia with 8{\%} oxygen at 2.5 atmospheres absolute (IR + hyperbaric normoxia, n = 5). Direct HBO treatment of neutrophils was also evaluated. Results: Neutrophils exposed to IR plasma showed a significant increase in percent adherent (0.8 ± 0.1{\%} versus 16.7 ± 2.2{\%}, P < 0.05) and polarized cells (6.2 ± 1.7{\%} versus 43.9 ± 12.2{\%}, P < 0.05) compared to controls. Hyperbaric oxygen significantly reduced the adhesion and polarization to 1.6 ± 0.3 and 4.1 ± 2.5{\%}, respectively (P = < 0.05). Normobaric hyperoxia and hyperbaric normoxia did not affect neutrophil adherence or CD18 polarization following IR. Direct HBO treatment of neutrophils did not change the percent of polarized cells in IR. Conclusions: Hyperbaric oxygen inhibits IR-induced neutrophil adhesion by blocking CD18 surface polarization and requires plasma exposure to HBO. Treatment with oxygen or pressure alone is not effective.",
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AU - Stephenson, Linda L.

AU - Nataraj, Chandra

AU - Wang, Wei Z.

AU - Zamboni, William A.

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