Requirement for engrailed and invected genes reveals novel regulatory interactions between engrailed/invected, patched, gooseberry and wingless during Drosophila neurogenesis

Krishna Moorthi Bhat, Paul Schedl

Research output: Contribution to journalArticle

53 Scopus citations

Abstract

During neurogenesis, the transmembrane protein Patched (Ptc) promotes a wingless (wg)-mediated specification of a neuronal precursor cell, NB4-2, by repressing gooseberry (gsb). In this study, novel interactions of these genes with engrailed (en) and invected (inv) during neurogenesis have been uncovered. While in row 4 cells Ptc represses gsb and wg, in row 5 cells en/inv relieve Ptc repression of gsb by a non-autonomous mechanism that does not involve hedgehog (hh). This differential regulation of gsb leads to the specification of NB5-3 and NB4-2 identities to two distinct neuroblasts. The uncoupling of the ptc-gsb regulatory circuit also enables gsb to promote Wg expression in row 5 cells. Our results suggest that the en/inv → ptc → gsb → wg pathway uncovered here and the hh → wg are distinct pathways that function to maintain wild-type level of Wg. Our results also indicate that Hh is not the only ligand for Ptc and similarly Ptc is not the only receptor for Hh.

Original languageEnglish (US)
Pages (from-to)1675-1688
Number of pages14
JournalDevelopment
Volume124
Issue number9
StatePublished - May 1 1997

Keywords

  • Drosophila
  • Engrailed
  • Gooseberry
  • Invected
  • Neurogenesis
  • Patched
  • Signaling

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology

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