Requirement for SNAPC1 in transcriptional responsiveness to diverse extracellular signals

David Baillat, Alessandro Gardini, Matteo Cesaroni, Ramin Shiekhattar

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Initiation of transcription of RNA polymerase II (RNAPII)-dependent genes requires the participation of a host of basal transcription factors. Among genes requiring RNAPII for transcription, small nuclear RNAs (snRNAs) display a further requirement for a factor known as snRNA-activating protein complex (SNAPc). The scope of the biological function of SNAPc and its requirement for transcription of protein-coding genes has not been elucidated. To determine the genome-wide occupancy of SNAPc, we performed chromatin immunoprecipitation followed by high-throughput sequencing using antibodies against SNAPC4 and SNAPC1 subunits. Interestingly, while SNAPC4 occupancy was limited to snRNA genes, SNAPC1 chromatin residence extended beyond snRNA genes to include a large number of transcriptionally active protein-coding genes. Notably, SNAPC1 occupancy on highly active genes mirrored that of elongating RNAPII extending through the bodies and 3′ ends of protein-coding genes. Inhibition of transcriptional elongation resulted in the loss of SNAPC1 from the 3′ ends of genes, reflecting a functional association between SNAPC1 and elongating RNAPII. Importantly, while depletion of SNAPC1 had a small effect on basal transcription, it diminished the transcriptional responsiveness of a large number of genes to two distinct extracellular stimuli, epidermal growth factor (EGF) and retinoic acid (RA). These results highlight a role for SNAPC1 as a general transcriptional coactivator that functions through elongating RNAPII.

Original languageEnglish (US)
Pages (from-to)4642-4650
Number of pages9
JournalMolecular and Cellular Biology
Volume32
Issue number22
DOIs
StatePublished - Nov 2012
Externally publishedYes

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Small Nuclear RNA
RNA Polymerase II
Genes
Proteins
Chromatin Immunoprecipitation
Tretinoin
Epidermal Growth Factor
Chromatin
Transcription Factors
Genome
Antibodies

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Requirement for SNAPC1 in transcriptional responsiveness to diverse extracellular signals. / Baillat, David; Gardini, Alessandro; Cesaroni, Matteo; Shiekhattar, Ramin.

In: Molecular and Cellular Biology, Vol. 32, No. 22, 11.2012, p. 4642-4650.

Research output: Contribution to journalArticle

Baillat, David ; Gardini, Alessandro ; Cesaroni, Matteo ; Shiekhattar, Ramin. / Requirement for SNAPC1 in transcriptional responsiveness to diverse extracellular signals. In: Molecular and Cellular Biology. 2012 ; Vol. 32, No. 22. pp. 4642-4650.
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