Requirement of DNA polymerase η for error-free bypass of UV-induced CC and TC photoproducts

S. L. Yu, R. E. Johnson, Satya Prakash, Louise Prakash

Research output: Contribution to journalArticle

110 Citations (Scopus)

Abstract

The yeast RAD30-encoded DNA polymerase η (Polη) bypasses a cis-syn thymine-thymine dimer efficiently and accurately. Human DNA polymerase η functions similarly in the bypass of this lesion, and mutations in human Polη result in the cancer prone syndrome, the variant form of xeroderma pigmentosum. UV light, however, also elicits the formation of cis-syn cyclobutane dimers and (6-4) photoproducts at 5'-CC-3' and 5'-TC-3' sites, and in both yeast and human DNA, UV-induced mutations occur primarily by 3' C to T transitions. Genetic studies presented here reveal a role for yeast Polη in the error-free bypass of cyclobutane dimers and (6-4) photoproducts formed at CC and TC sites. Thus, by preventing UV mutagenesis at a wide spectrum of dipyrimidine sites, Polη plays a pivotal role in minimizing the incidence of sunlight-induced skin cancers in humans.

Original languageEnglish (US)
Pages (from-to)185-188
Number of pages4
JournalMolecular and Cellular Biology
Volume21
Issue number1
DOIs
StatePublished - 2001

Fingerprint

DNA-Directed DNA Polymerase
Cyclobutanes
Yeasts
Pyrimidine Dimers
Mutation
Thymine
Sunlight
Skin Neoplasms
Ultraviolet Rays
Mutagenesis
DNA
Incidence
Neoplasms

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Requirement of DNA polymerase η for error-free bypass of UV-induced CC and TC photoproducts. / Yu, S. L.; Johnson, R. E.; Prakash, Satya; Prakash, Louise.

In: Molecular and Cellular Biology, Vol. 21, No. 1, 2001, p. 185-188.

Research output: Contribution to journalArticle

@article{f0f821412639408dbf6ec528b16f08b6,
title = "Requirement of DNA polymerase η for error-free bypass of UV-induced CC and TC photoproducts",
abstract = "The yeast RAD30-encoded DNA polymerase η (Polη) bypasses a cis-syn thymine-thymine dimer efficiently and accurately. Human DNA polymerase η functions similarly in the bypass of this lesion, and mutations in human Polη result in the cancer prone syndrome, the variant form of xeroderma pigmentosum. UV light, however, also elicits the formation of cis-syn cyclobutane dimers and (6-4) photoproducts at 5'-CC-3' and 5'-TC-3' sites, and in both yeast and human DNA, UV-induced mutations occur primarily by 3' C to T transitions. Genetic studies presented here reveal a role for yeast Polη in the error-free bypass of cyclobutane dimers and (6-4) photoproducts formed at CC and TC sites. Thus, by preventing UV mutagenesis at a wide spectrum of dipyrimidine sites, Polη plays a pivotal role in minimizing the incidence of sunlight-induced skin cancers in humans.",
author = "Yu, {S. L.} and Johnson, {R. E.} and Satya Prakash and Louise Prakash",
year = "2001",
doi = "10.1128/MCB.21.1.185-188.2001",
language = "English (US)",
volume = "21",
pages = "185--188",
journal = "Molecular and Cellular Biology",
issn = "0270-7306",
publisher = "American Society for Microbiology",
number = "1",

}

TY - JOUR

T1 - Requirement of DNA polymerase η for error-free bypass of UV-induced CC and TC photoproducts

AU - Yu, S. L.

AU - Johnson, R. E.

AU - Prakash, Satya

AU - Prakash, Louise

PY - 2001

Y1 - 2001

N2 - The yeast RAD30-encoded DNA polymerase η (Polη) bypasses a cis-syn thymine-thymine dimer efficiently and accurately. Human DNA polymerase η functions similarly in the bypass of this lesion, and mutations in human Polη result in the cancer prone syndrome, the variant form of xeroderma pigmentosum. UV light, however, also elicits the formation of cis-syn cyclobutane dimers and (6-4) photoproducts at 5'-CC-3' and 5'-TC-3' sites, and in both yeast and human DNA, UV-induced mutations occur primarily by 3' C to T transitions. Genetic studies presented here reveal a role for yeast Polη in the error-free bypass of cyclobutane dimers and (6-4) photoproducts formed at CC and TC sites. Thus, by preventing UV mutagenesis at a wide spectrum of dipyrimidine sites, Polη plays a pivotal role in minimizing the incidence of sunlight-induced skin cancers in humans.

AB - The yeast RAD30-encoded DNA polymerase η (Polη) bypasses a cis-syn thymine-thymine dimer efficiently and accurately. Human DNA polymerase η functions similarly in the bypass of this lesion, and mutations in human Polη result in the cancer prone syndrome, the variant form of xeroderma pigmentosum. UV light, however, also elicits the formation of cis-syn cyclobutane dimers and (6-4) photoproducts at 5'-CC-3' and 5'-TC-3' sites, and in both yeast and human DNA, UV-induced mutations occur primarily by 3' C to T transitions. Genetic studies presented here reveal a role for yeast Polη in the error-free bypass of cyclobutane dimers and (6-4) photoproducts formed at CC and TC sites. Thus, by preventing UV mutagenesis at a wide spectrum of dipyrimidine sites, Polη plays a pivotal role in minimizing the incidence of sunlight-induced skin cancers in humans.

UR - http://www.scopus.com/inward/record.url?scp=0034747804&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034747804&partnerID=8YFLogxK

U2 - 10.1128/MCB.21.1.185-188.2001

DO - 10.1128/MCB.21.1.185-188.2001

M3 - Article

VL - 21

SP - 185

EP - 188

JO - Molecular and Cellular Biology

JF - Molecular and Cellular Biology

SN - 0270-7306

IS - 1

ER -