TY - JOUR
T1 - Requirement of DNA polymerase η for error-free bypass of UV-induced CC and TC photoproducts
AU - Yu, S. L.
AU - Johnson, R. E.
AU - Prakash, S.
AU - Prakash, L.
PY - 2001
Y1 - 2001
N2 - The yeast RAD30-encoded DNA polymerase η (Polη) bypasses a cis-syn thymine-thymine dimer efficiently and accurately. Human DNA polymerase η functions similarly in the bypass of this lesion, and mutations in human Polη result in the cancer prone syndrome, the variant form of xeroderma pigmentosum. UV light, however, also elicits the formation of cis-syn cyclobutane dimers and (6-4) photoproducts at 5'-CC-3' and 5'-TC-3' sites, and in both yeast and human DNA, UV-induced mutations occur primarily by 3' C to T transitions. Genetic studies presented here reveal a role for yeast Polη in the error-free bypass of cyclobutane dimers and (6-4) photoproducts formed at CC and TC sites. Thus, by preventing UV mutagenesis at a wide spectrum of dipyrimidine sites, Polη plays a pivotal role in minimizing the incidence of sunlight-induced skin cancers in humans.
AB - The yeast RAD30-encoded DNA polymerase η (Polη) bypasses a cis-syn thymine-thymine dimer efficiently and accurately. Human DNA polymerase η functions similarly in the bypass of this lesion, and mutations in human Polη result in the cancer prone syndrome, the variant form of xeroderma pigmentosum. UV light, however, also elicits the formation of cis-syn cyclobutane dimers and (6-4) photoproducts at 5'-CC-3' and 5'-TC-3' sites, and in both yeast and human DNA, UV-induced mutations occur primarily by 3' C to T transitions. Genetic studies presented here reveal a role for yeast Polη in the error-free bypass of cyclobutane dimers and (6-4) photoproducts formed at CC and TC sites. Thus, by preventing UV mutagenesis at a wide spectrum of dipyrimidine sites, Polη plays a pivotal role in minimizing the incidence of sunlight-induced skin cancers in humans.
UR - http://www.scopus.com/inward/record.url?scp=0034747804&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034747804&partnerID=8YFLogxK
U2 - 10.1128/MCB.21.1.185-188.2001
DO - 10.1128/MCB.21.1.185-188.2001
M3 - Article
C2 - 11113193
AN - SCOPUS:0034747804
SN - 0270-7306
VL - 21
SP - 185
EP - 188
JO - Molecular and Cellular Biology
JF - Molecular and Cellular Biology
IS - 1
ER -