Abstract
Tumor cells strategically down-regulate Fas receptor expression to evade immune attack and up-regulate expression of Fas ligand to promote apoptosis of in filtrating T lymphocytes. Many pathways leading to apoptotic cell death require calcium release from inositol 1,4,5-trisphosphate receptors (IP 3Rs). Here, we show that Fas-dependent killing of Jurkat T lymphoma cells by SW620 colon cancer cells requires calcium release from IP3R. General suppression of IP3R signaling significantly reduced SW620-mediated Jurkat cell apoptosis. Significantly, a specific inhibitor of apoptotic calcium release from IP3R strongly blocked lymphocyte apoptosis. Thus, selective pharmacological targeting of apoptotic calcium release from IP3R may enhance tumor cell immunogenicity.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 13506-13509 |
| Number of pages | 4 |
| Journal | Journal of Biological Chemistry |
| Volume | 283 |
| Issue number | 20 |
| DOIs | |
| State | Published - May 16 2008 |
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ASJC Scopus subject areas
- Biochemistry
- Cell Biology
- Molecular Biology
Cite this
Requirement of inositol 1,4,5-trisphosphate receptors for tumor-mediated lymphocyte apoptosis. / Steinmann, Camia; Landsverk, Megan L.; Barral, José M.; Boehning, Darren.
In: Journal of Biological Chemistry, Vol. 283, No. 20, 16.05.2008, p. 13506-13509.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Requirement of inositol 1,4,5-trisphosphate receptors for tumor-mediated lymphocyte apoptosis
AU - Steinmann, Camia
AU - Landsverk, Megan L.
AU - Barral, José M.
AU - Boehning, Darren
PY - 2008/5/16
Y1 - 2008/5/16
N2 - Tumor cells strategically down-regulate Fas receptor expression to evade immune attack and up-regulate expression of Fas ligand to promote apoptosis of in filtrating T lymphocytes. Many pathways leading to apoptotic cell death require calcium release from inositol 1,4,5-trisphosphate receptors (IP 3Rs). Here, we show that Fas-dependent killing of Jurkat T lymphoma cells by SW620 colon cancer cells requires calcium release from IP3R. General suppression of IP3R signaling significantly reduced SW620-mediated Jurkat cell apoptosis. Significantly, a specific inhibitor of apoptotic calcium release from IP3R strongly blocked lymphocyte apoptosis. Thus, selective pharmacological targeting of apoptotic calcium release from IP3R may enhance tumor cell immunogenicity.
AB - Tumor cells strategically down-regulate Fas receptor expression to evade immune attack and up-regulate expression of Fas ligand to promote apoptosis of in filtrating T lymphocytes. Many pathways leading to apoptotic cell death require calcium release from inositol 1,4,5-trisphosphate receptors (IP 3Rs). Here, we show that Fas-dependent killing of Jurkat T lymphoma cells by SW620 colon cancer cells requires calcium release from IP3R. General suppression of IP3R signaling significantly reduced SW620-mediated Jurkat cell apoptosis. Significantly, a specific inhibitor of apoptotic calcium release from IP3R strongly blocked lymphocyte apoptosis. Thus, selective pharmacological targeting of apoptotic calcium release from IP3R may enhance tumor cell immunogenicity.
UR - http://www.scopus.com/inward/record.url?scp=46649097078&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=46649097078&partnerID=8YFLogxK
U2 - 10.1074/jbc.C800029200
DO - 10.1074/jbc.C800029200
M3 - Article
C2 - 18364356
AN - SCOPUS:46649097078
VL - 283
SP - 13506
EP - 13509
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
SN - 0021-9258
IS - 20
ER -