Requirement of the yeast RTH1 5′ to 3′ exonuclease for the stability of simple repetitive DNA

Robert E. Johnson, Gopala K. Kovvali, Louise Prakash, Satya Prakash

Research output: Contribution to journalArticle

191 Citations (Scopus)

Abstract

Simple repetitive DNA sequences are unstable in human colorectal cancers and a variety of other cancers. Mutations in the DNA mismatch repair genes MSH2, MLH1, and PMS1 result in elevated rates of spontaneous mutation and cause a marked increase in the instability of simple repeats. Compared with the wild type, a null mutation in the yeast RTH1 gene, which encodes a 5′ to 3′ exonuclease, was shown to increase the rate of instability of simple repetitive DNA by as much as 280 times and to increase the spontaneous mutation rate by 30 times. Epistasis analyses were consistent with the hypothesis that this RTH1-encoded nuclease has a role in the MSH2-MLH1-PMS1 mismatch repair pathway.

Original languageEnglish
Pages (from-to)238-240
Number of pages3
JournalScience
Volume269
Issue number5221
StatePublished - Jul 14 1995

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Exonucleases
DNA Mismatch Repair
Mutation Rate
Yeasts
Mutation
DNA
Microsatellite Repeats
Genes
Colorectal Neoplasms
Neoplasms

ASJC Scopus subject areas

  • General

Cite this

Requirement of the yeast RTH1 5′ to 3′ exonuclease for the stability of simple repetitive DNA. / Johnson, Robert E.; Kovvali, Gopala K.; Prakash, Louise; Prakash, Satya.

In: Science, Vol. 269, No. 5221, 14.07.1995, p. 238-240.

Research output: Contribution to journalArticle

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