Requirement of yeast SGS1 and SRS2 genes for replication and transcription

Sung Keun Lee, Robert E. Johnson, Sung Lim Yu, Louise Prakash, Satya Prakash

Research output: Contribution to journalArticle

120 Citations (Scopus)

Abstract

The SGS1 gene of the yeast Saccharormyces cerevisiae encodes a DNA helicase with homology to the human Bloom's syndrome gene BLM and the Werner's syndrome gene WRN. The SRS2 gene of yeast also encodes a DNA helicase. Simultaneous deletion of SGS1 and SRS2 is lethal in yeast. Here, using a conditional mutation of SGS1, it is shown that DNA replication and RNA polymerase 1 transcription are drastically inhibited in the srs2Δ sgs1- ts strain at the restrictive temperature. Thus, SGS1 and SRS2 function in DNA replication and RNA polymerase 1 transcription. These functions may contribute to the various defects observed in Werner's and Bloom's syndromes.

Original languageEnglish (US)
Pages (from-to)2339-2342
Number of pages4
JournalScience
Volume286
Issue number5448
DOIs
StatePublished - Dec 17 1999
Externally publishedYes

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Yeasts
Bloom Syndrome
Werner Syndrome
DNA Helicases
DNA-Directed DNA Polymerase
DNA-Directed RNA Polymerases
DNA Replication
Genes
Mutation
Temperature

ASJC Scopus subject areas

  • General

Cite this

Requirement of yeast SGS1 and SRS2 genes for replication and transcription. / Lee, Sung Keun; Johnson, Robert E.; Yu, Sung Lim; Prakash, Louise; Prakash, Satya.

In: Science, Vol. 286, No. 5448, 17.12.1999, p. 2339-2342.

Research output: Contribution to journalArticle

Lee, Sung Keun ; Johnson, Robert E. ; Yu, Sung Lim ; Prakash, Louise ; Prakash, Satya. / Requirement of yeast SGS1 and SRS2 genes for replication and transcription. In: Science. 1999 ; Vol. 286, No. 5448. pp. 2339-2342.
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