TY - JOUR
T1 - Requirements for indispensable amino acids in adult humans
T2 - Longer-term amino acid kinetic study with support for the adequacy of the Massachusetts Institute of Technology amino acid requirement pattern
AU - Marchini, J. S.
AU - Cortiella, J.
AU - Hiramatsu, T.
AU - Chapman, T. E.
AU - Young, V. R.
PY - 1993
Y1 - 1993
N2 - Twenty young men received an L-amino acid diet, supplying 140 mg N · kg- 1 · d-1 and patterned as in the Egg diet for 1 wk, and then for 3 wk either a pattern based on international recommendations (modified FAO diet; n = 7), our new amino acid requirement pattern (MIT diet; n = 7), or the egg pattern (Egg diet; n = 6). At the end of the initial week, at 1 and 3 wk with the three experimental diets, and after 3 d after return to the Egg diet, an 8-h continuous intravenous infusion with [1-13C]leucine (3 h fast, 5 h fed while subjects received hourly meals supplying the equivalent of one-twelfth the daily intake) was conducted. After 3 wk with the different diets, mean daily leucine balances were lower (P < 0.01) with the FAO diet (-160 μmol · kg-1 · d-1) than with the MIT diet (-15 μmol · kg-1 · d-1). Together with changes in plasma amino acid profiles [eg, methionine increased (P < 0.05) during feeding with the FAO and Egg diets but not with the MIT diet; increased proline concentrations during the fed state (P < 0.05) with the FAO diet but not with the MIT or Egg diets] we interpret these findings to indicate that the FAO diet is not capable of maintaining amino acid homeostasis, as is the case with the MIT and Egg diets.
AB - Twenty young men received an L-amino acid diet, supplying 140 mg N · kg- 1 · d-1 and patterned as in the Egg diet for 1 wk, and then for 3 wk either a pattern based on international recommendations (modified FAO diet; n = 7), our new amino acid requirement pattern (MIT diet; n = 7), or the egg pattern (Egg diet; n = 6). At the end of the initial week, at 1 and 3 wk with the three experimental diets, and after 3 d after return to the Egg diet, an 8-h continuous intravenous infusion with [1-13C]leucine (3 h fast, 5 h fed while subjects received hourly meals supplying the equivalent of one-twelfth the daily intake) was conducted. After 3 wk with the different diets, mean daily leucine balances were lower (P < 0.01) with the FAO diet (-160 μmol · kg-1 · d-1) than with the MIT diet (-15 μmol · kg-1 · d-1). Together with changes in plasma amino acid profiles [eg, methionine increased (P < 0.05) during feeding with the FAO and Egg diets but not with the MIT diet; increased proline concentrations during the fed state (P < 0.05) with the FAO diet but not with the MIT or Egg diets] we interpret these findings to indicate that the FAO diet is not capable of maintaining amino acid homeostasis, as is the case with the MIT and Egg diets.
KW - Amino acid requirements
KW - healthy adult men
KW - leucine
KW - nitrogen balance
KW - oxidation balance
KW - plasma amino acids
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U2 - 10.1093/ajcn/58.5.670
DO - 10.1093/ajcn/58.5.670
M3 - Article
C2 - 8237874
AN - SCOPUS:0027427402
VL - 58
SP - 670
EP - 683
JO - American Journal of Clinical Nutrition
JF - American Journal of Clinical Nutrition
SN - 0002-9165
IS - 5
ER -