Abstract
Twenty young men received an L-amino acid diet, supplying 140 mg N·kg-1·d-1 and patterned as in the Egg diet for 1 wk, and then for 3 wk either a pattern based on international recommendations (modified FAO diet; n = 7), our new amino acid requirement pattern (MIT diet; n = 7), or the egg pattern (Egg diet; n = 6). At the end of the initial week, at 1 and 3 wk with the three experimental diets, and after 3 d after return to the Egg diet, an 8-h continuous intravenous infusion with [1-13C]leucine (3 h fast, 5 h fed while subjects received hourly meals supplying the equivalent of one-twelfth the daily intake) was conducted. After 3 wk with the different diets, mean daily leucine balances were lower (P < 0.01) with the FAO diet (-160 μmol·kg-1·d-1) than with the MIT diet (-15 μmol·kg-1·d-1). Together with changes in plasma amino acid profiles [eg, methionine increased (P < 0.05) during feeding with the FAO and Egg diets but not with the MIT diet; increased proline concentrations during the fed state (P < 0.05) with the FAO diet but not with the MIT or Egg diets] we interpret these findings to indicate that the FAO diet is not capable of maintaining amino acid homeostasis, as is the case with the MIT and Egg diets.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 670-683 |
| Number of pages | 14 |
| Journal | American Journal of Clinical Nutrition |
| Volume | 58 |
| Issue number | 5 |
| State | Published - Nov 1993 |
| Externally published | Yes |
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Keywords
- Amino acid requirements
- Healthy adult men
- Leucine
- Nitrogen balance
- Oxidation balance
- Plasma amino acids
ASJC Scopus subject areas
- Food Science
- Medicine (miscellaneous)
Cite this
Requirements for indispensable amino acids in adult humans : Longer-term amino acid kinetic study with support for the adequacy of the Massachusetts Institute of Technology amino acid requirement pattern. / Marchini, J. Sergio; Cortiella, Joaquin; Hiramatsu, Takeyuki; Chapman, Thomas E.; Young, Vernon R.
In: American Journal of Clinical Nutrition, Vol. 58, No. 5, 11.1993, p. 670-683.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Requirements for indispensable amino acids in adult humans
T2 - Longer-term amino acid kinetic study with support for the adequacy of the Massachusetts Institute of Technology amino acid requirement pattern
AU - Marchini, J. Sergio
AU - Cortiella, Joaquin
AU - Hiramatsu, Takeyuki
AU - Chapman, Thomas E.
AU - Young, Vernon R.
PY - 1993/11
Y1 - 1993/11
N2 - Twenty young men received an L-amino acid diet, supplying 140 mg N·kg-1·d-1 and patterned as in the Egg diet for 1 wk, and then for 3 wk either a pattern based on international recommendations (modified FAO diet; n = 7), our new amino acid requirement pattern (MIT diet; n = 7), or the egg pattern (Egg diet; n = 6). At the end of the initial week, at 1 and 3 wk with the three experimental diets, and after 3 d after return to the Egg diet, an 8-h continuous intravenous infusion with [1-13C]leucine (3 h fast, 5 h fed while subjects received hourly meals supplying the equivalent of one-twelfth the daily intake) was conducted. After 3 wk with the different diets, mean daily leucine balances were lower (P < 0.01) with the FAO diet (-160 μmol·kg-1·d-1) than with the MIT diet (-15 μmol·kg-1·d-1). Together with changes in plasma amino acid profiles [eg, methionine increased (P < 0.05) during feeding with the FAO and Egg diets but not with the MIT diet; increased proline concentrations during the fed state (P < 0.05) with the FAO diet but not with the MIT or Egg diets] we interpret these findings to indicate that the FAO diet is not capable of maintaining amino acid homeostasis, as is the case with the MIT and Egg diets.
AB - Twenty young men received an L-amino acid diet, supplying 140 mg N·kg-1·d-1 and patterned as in the Egg diet for 1 wk, and then for 3 wk either a pattern based on international recommendations (modified FAO diet; n = 7), our new amino acid requirement pattern (MIT diet; n = 7), or the egg pattern (Egg diet; n = 6). At the end of the initial week, at 1 and 3 wk with the three experimental diets, and after 3 d after return to the Egg diet, an 8-h continuous intravenous infusion with [1-13C]leucine (3 h fast, 5 h fed while subjects received hourly meals supplying the equivalent of one-twelfth the daily intake) was conducted. After 3 wk with the different diets, mean daily leucine balances were lower (P < 0.01) with the FAO diet (-160 μmol·kg-1·d-1) than with the MIT diet (-15 μmol·kg-1·d-1). Together with changes in plasma amino acid profiles [eg, methionine increased (P < 0.05) during feeding with the FAO and Egg diets but not with the MIT diet; increased proline concentrations during the fed state (P < 0.05) with the FAO diet but not with the MIT or Egg diets] we interpret these findings to indicate that the FAO diet is not capable of maintaining amino acid homeostasis, as is the case with the MIT and Egg diets.
KW - Amino acid requirements
KW - Healthy adult men
KW - Leucine
KW - Nitrogen balance
KW - Oxidation balance
KW - Plasma amino acids
UR - http://www.scopus.com/inward/record.url?scp=0027427402&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027427402&partnerID=8YFLogxK
M3 - Article
C2 - 8237874
AN - SCOPUS:0027427402
VL - 58
SP - 670
EP - 683
JO - American Journal of Clinical Nutrition
JF - American Journal of Clinical Nutrition
SN - 0002-9165
IS - 5
ER -