Rescue of a recombinant Machupo virus from cloned cDNAs and in vivo characterization in interferon (αβ/γ) receptor double knockout mice

Michael Patterson, Alexey Seregin, Cheng Huang, Olga Kolokoltsova, Jennifer Smith, Milagros Miller, Jeanon Smith, Nadezhda E Yun, Allison Poussard, Ashley Grant, Bersabeh Tigabu, Aida Walker, Slobodan Paessler

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

Machupo virus (MACV) is the etiological agent of Bolivian hemorrhagic fever (BHF), a reemerging and neglected tropical disease associated with high mortality. The prototypical strain of MACV, Carvallo, was isolated from a human patient in 1963, but minimal in vitro and in vivo characterization has been reported. To this end, we utilized reverse genetics to rescue a pathogenic MACV from cloned cDNAs. The recombinant MACV (rMACV) had in vitro growth properties similar to those of the parental MACV. Both viruses caused similar disease development in alpha/beta and gamma interferon receptor knockout mice, including neurological disease development and high mortality. In addition, we have identified a novel murine model with mortality and neurological disease similar to BHF disease reported in humans and nonhuman primates.

Original languageEnglish (US)
Pages (from-to)1914-1923
Number of pages10
JournalJournal of virology
Volume88
Issue number4
DOIs
StatePublished - Feb 2014

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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    Patterson, M., Seregin, A., Huang, C., Kolokoltsova, O., Smith, J., Miller, M., Smith, J., Yun, N. E., Poussard, A., Grant, A., Tigabu, B., Walker, A., & Paessler, S. (2014). Rescue of a recombinant Machupo virus from cloned cDNAs and in vivo characterization in interferon (αβ/γ) receptor double knockout mice. Journal of virology, 88(4), 1914-1923. https://doi.org/10.1128/JVI.02925-13