Rescue of a recombinant Machupo virus from cloned cDNAs and in vivo characterization in interferon (αβ/γ) receptor double knockout mice

Michael Patterson, Alexey Seregin, Cheng Huang, Olga Kolokoltsova, Jennifer Smith, Milagros Miller, Jeanon Smith, Nadezhda Yun, Allison Poussard, Ashley Grant, Bersabeh Tigabu, Aida Walker, Slobodan Paessler

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Machupo virus (MACV) is the etiological agent of Bolivian hemorrhagic fever (BHF), a reemerging and neglected tropical disease associated with high mortality. The prototypical strain of MACV, Carvallo, was isolated from a human patient in 1963, but minimal in vitro and in vivo characterization has been reported. To this end, we utilized reverse genetics to rescue a pathogenic MACV from cloned cDNAs. The recombinant MACV (rMACV) had in vitro growth properties similar to those of the parental MACV. Both viruses caused similar disease development in alpha/beta and gamma interferon receptor knockout mice, including neurological disease development and high mortality. In addition, we have identified a novel murine model with mortality and neurological disease similar to BHF disease reported in humans and nonhuman primates.

Original languageEnglish (US)
Pages (from-to)1914-1923
Number of pages10
JournalJournal of Virology
Volume88
Issue number4
DOIs
StatePublished - Feb 2014

Fingerprint

Machupo virus
New World Arenaviruses
Interferon Receptors
interferons
Knockout Mice
Complementary DNA
American Hemorrhagic Fever
receptors
mice
fever
Mortality
Neglected Diseases
Reverse Genetics
Interferon-beta
etiological agents
interferon-gamma
human diseases
Interferon-alpha
Primates
Interferon-gamma

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Rescue of a recombinant Machupo virus from cloned cDNAs and in vivo characterization in interferon (αβ/γ) receptor double knockout mice. / Patterson, Michael; Seregin, Alexey; Huang, Cheng; Kolokoltsova, Olga; Smith, Jennifer; Miller, Milagros; Smith, Jeanon; Yun, Nadezhda; Poussard, Allison; Grant, Ashley; Tigabu, Bersabeh; Walker, Aida; Paessler, Slobodan.

In: Journal of Virology, Vol. 88, No. 4, 02.2014, p. 1914-1923.

Research output: Contribution to journalArticle

Patterson, M, Seregin, A, Huang, C, Kolokoltsova, O, Smith, J, Miller, M, Smith, J, Yun, N, Poussard, A, Grant, A, Tigabu, B, Walker, A & Paessler, S 2014, 'Rescue of a recombinant Machupo virus from cloned cDNAs and in vivo characterization in interferon (αβ/γ) receptor double knockout mice', Journal of Virology, vol. 88, no. 4, pp. 1914-1923. https://doi.org/10.1128/JVI.02925-13
Patterson, Michael ; Seregin, Alexey ; Huang, Cheng ; Kolokoltsova, Olga ; Smith, Jennifer ; Miller, Milagros ; Smith, Jeanon ; Yun, Nadezhda ; Poussard, Allison ; Grant, Ashley ; Tigabu, Bersabeh ; Walker, Aida ; Paessler, Slobodan. / Rescue of a recombinant Machupo virus from cloned cDNAs and in vivo characterization in interferon (αβ/γ) receptor double knockout mice. In: Journal of Virology. 2014 ; Vol. 88, No. 4. pp. 1914-1923.
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