Residence Times of Molecular Complexes in Solution from NMR Data of Intermolecular Hydrogen-Bond Scalar Coupling

Levani Zandarashvili, Alexandre Esadze, Catherine A. Kemme, Abhijnan Chattopadhyay, Dan Nguyen, Junji Iwahara

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The residence times of molecular complexes in solution are important for understanding biomolecular functions and drug actions. We show that NMR data of intermolecular hydrogen-bond scalar couplings can yield information on the residence times of molecular complexes in solution. The molecular exchange of binding partners via the breakage and reformation of a complex causes self-decoupling of intermolecular hydrogen-bond scalar couplings, and this self-decoupling effect depends on the residence time of the complex. For protein-DNA complexes, we investigated the salt concentration dependence of intermolecular hydrogen-bond scalar couplings between the protein side-chain 15N and DNA phosphate 31P nuclei, from which the residence times were analyzed. The results were consistent with those obtained by 15Nz-exchange spectroscopy. This self-decoupling-based kinetic analysis is unique in that it does not require any different signatures for the states involved in the exchange, whereas such conditions are crucial for kinetic analyses by typical NMR and other methods.

Original languageEnglish (US)
Pages (from-to)820-824
Number of pages5
JournalJournal of Physical Chemistry Letters
Volume7
Issue number5
DOIs
StatePublished - Mar 3 2016

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Hydrogen bonds
Nuclear magnetic resonance
decoupling
hydrogen bonds
scalars
nuclear magnetic resonance
DNA
Proteins
Kinetics
deoxyribonucleic acid
proteins
Ion exchange
kinetics
Phosphates
Salts
Spectroscopy
phosphates
drugs
signatures
salts

ASJC Scopus subject areas

  • Materials Science(all)

Cite this

Residence Times of Molecular Complexes in Solution from NMR Data of Intermolecular Hydrogen-Bond Scalar Coupling. / Zandarashvili, Levani; Esadze, Alexandre; Kemme, Catherine A.; Chattopadhyay, Abhijnan; Nguyen, Dan; Iwahara, Junji.

In: Journal of Physical Chemistry Letters, Vol. 7, No. 5, 03.03.2016, p. 820-824.

Research output: Contribution to journalArticle

Zandarashvili, Levani ; Esadze, Alexandre ; Kemme, Catherine A. ; Chattopadhyay, Abhijnan ; Nguyen, Dan ; Iwahara, Junji. / Residence Times of Molecular Complexes in Solution from NMR Data of Intermolecular Hydrogen-Bond Scalar Coupling. In: Journal of Physical Chemistry Letters. 2016 ; Vol. 7, No. 5. pp. 820-824.
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