Residence Times of Molecular Complexes in Solution from NMR Data of Intermolecular Hydrogen-Bond Scalar Coupling

Levani Zandarashvili, Alexandre Esadze, Catherine A. Kemme, Abhijnan Chattopadhyay, Dan Nguyen, Junji Iwahara

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

The residence times of molecular complexes in solution are important for understanding biomolecular functions and drug actions. We show that NMR data of intermolecular hydrogen-bond scalar couplings can yield information on the residence times of molecular complexes in solution. The molecular exchange of binding partners via the breakage and reformation of a complex causes self-decoupling of intermolecular hydrogen-bond scalar couplings, and this self-decoupling effect depends on the residence time of the complex. For protein-DNA complexes, we investigated the salt concentration dependence of intermolecular hydrogen-bond scalar couplings between the protein side-chain 15N and DNA phosphate 31P nuclei, from which the residence times were analyzed. The results were consistent with those obtained by 15Nz-exchange spectroscopy. This self-decoupling-based kinetic analysis is unique in that it does not require any different signatures for the states involved in the exchange, whereas such conditions are crucial for kinetic analyses by typical NMR and other methods.

Original languageEnglish (US)
Pages (from-to)820-824
Number of pages5
JournalJournal of Physical Chemistry Letters
Volume7
Issue number5
DOIs
StatePublished - Mar 3 2016

    Fingerprint

ASJC Scopus subject areas

  • Materials Science(all)

Cite this