Respiratory syncytial virus F envelope protein associates with lipid rafts without a requirement for other virus proteins

Elisa H. Fleming, Andrey A. Kolokoltsov, Robert A. Davey, Joan Nichols, Norbert J. Roberts

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Like many enveloped viruses, human respiratory syncytial virus (RSV) assembles at and buds from lipid rafts. Translocation of the envelope proteins to these membrane subdomains is essential for production of infectious virus, but the targeting mechanism is poorly understood and it is not known if other virus proteins are required. Here we demonstrate that F protein of RSV intrinsically targets to lipid rafts without a requirement for any other virus protein, including the SH and G envelope proteins. Recombinant virus deficient in SH and G but retaining F protein expression was used to demonstrate that F protein still localized in rafts in both A549 and HEp-2 cells. Expression of a recombinant F gene by use of plasmid vectors demonstrated that F contains its own targeting domain and localized to rafts in the absence of other virus proteins. The domain responsible for translocation was then mapped. Unlike most other virus envelope proteins, F is unusual since the target signal is not contained within the cytoplasmic domain nor did it involve fatty acid modified residues. Furthermore, exchange of the transmembrane domain with that of the vesicular stomatitis virus G protein, a nonraft protein, did not alter F protein raft localization. Taken together, these data suggest that domains present in the extracellular portion of the protein are responsible for lipid raft targeting of the RSV F protein.

Original languageEnglish (US)
Pages (from-to)12160-12170
Number of pages11
JournalJournal of Virology
Volume80
Issue number24
DOIs
StatePublished - Dec 2006

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Viral Envelope Proteins
Respiratory Syncytial Viruses
Viruses
Lipids
viruses
lipids
Proteins
proteins
Human respiratory syncytial virus
Vesiculovirus
plasmid vectors
Protein Transport
G-proteins
Membrane Proteins
Plasmids
Fatty Acids

ASJC Scopus subject areas

  • Immunology

Cite this

Respiratory syncytial virus F envelope protein associates with lipid rafts without a requirement for other virus proteins. / Fleming, Elisa H.; Kolokoltsov, Andrey A.; Davey, Robert A.; Nichols, Joan; Roberts, Norbert J.

In: Journal of Virology, Vol. 80, No. 24, 12.2006, p. 12160-12170.

Research output: Contribution to journalArticle

Fleming, Elisa H. ; Kolokoltsov, Andrey A. ; Davey, Robert A. ; Nichols, Joan ; Roberts, Norbert J. / Respiratory syncytial virus F envelope protein associates with lipid rafts without a requirement for other virus proteins. In: Journal of Virology. 2006 ; Vol. 80, No. 24. pp. 12160-12170.
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