Respiratory Syncytial Virus Infection Changes Cargo Composition of Exosome Released from Airway Epithelial Cells

Harendra Singh Chahar, Tiziana Gorgun, Andrzej S. Kudlicki, Narayana Komaravelli, Antonella Casola

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Exosomes are microvesicles known to carry biologically active molecules, including RNA, DNA and proteins. Viral infections can induce profound changes in exosome composition, and exosomes have been implicated in viral transmission and pathogenesis. No information is current available regarding exosome composition and function during infection with Respiratory Syncytial Virus (RSV), the most important cause of lower respiratory tract infections in children. In this study, we characterized exosomes released from RSV-infected lung carcinoma-derived A549 cells. RNA deep sequencing revealed that RSV exosomes contain a diverse range of RNA species like messenger and ribosomal RNA fragments, as well as small noncoding RNAs, in a proportion different from exosomes isolated from mock-infected cells. We observed that both RNA and protein signatures of RSV were present in exosomes, however, they were not able to establish productive infection in uninfected cells. Exosomes isolated from RSV-infected cells were able to activate innate immune response by inducing cytokine and chemokine release from human monocytes and airway epithelial cells. These data suggest that exosomes may play an important role in pathogenesis or protection against disease, therefore understating their role in RSV infection may open new avenues for target identification and development of novel therapeutics.

Original languageEnglish (US)
Article number387
JournalScientific Reports
Volume8
Issue number1
DOIs
StatePublished - Dec 1 2018

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Exosomes
Respiratory Syncytial Virus Infections
Epithelial Cells
Respiratory Syncytial Viruses
RNA
RNA Sequence Analysis
High-Throughput Nucleotide Sequencing
Small Untranslated RNA
Ribosomal RNA
Virus Diseases
Chemokines
Innate Immunity
Respiratory Tract Infections
Monocytes
Proteins
Cytokines

ASJC Scopus subject areas

  • General

Cite this

Respiratory Syncytial Virus Infection Changes Cargo Composition of Exosome Released from Airway Epithelial Cells. / Chahar, Harendra Singh; Gorgun, Tiziana; Kudlicki, Andrzej S.; Komaravelli, Narayana; Casola, Antonella.

In: Scientific Reports, Vol. 8, No. 1, 387, 01.12.2018.

Research output: Contribution to journalArticle

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