Respiratory syncytial virus infection in infants is associated with predominant Th-2-1ike response

Mariana Román, William Calhoun, Kim L. Hinton, Luis F. Avendaño, Valeska Simon, Ana M. Escobar, Aldo Gaggero, Patricia V. Díaz

Research output: Contribution to journalArticle

246 Citations (Scopus)

Abstract

Viral infections have been associated with cellular immune responses and production of Th-1 cytokines. Respiratory syncytial virus (RSV), however, induces virus-specific IgE, which might be a consequence of a Th-2-like activation. To test this hypothesis we quantified interferon-γ (IFN-γ) and interleukin-4 (IL-4) in the supernatant of peripheral blood mononuclear cells cultured for 24 and 48 h in the presence or absence of phytohemaglutinin and pokeweed mitogen and the lymphocyte phenotypes to analyze subsets and their activation markers, from 15 hospitalized infants during an acute lower respiratory infection caused by RSV and 17 healthy control infants from 1 to 15 mo of age. Compared with the control infants, those infected with RSV had an increase in the number of B-cells (p < 0.02) and decreases in both CD8+ T- cells (p < 0.01) and activated CD8+/CD25+ suppressor/cytotoxic T-cells (p < 0.007). In RSV-infected infants, IFN-γ production was subtotally suppressed, whereas IL-4 production was decreased to a lesser degree, giving significantly (p < 0.001) increased IL-4/IFN-γ ratio compared with that in the control infants. These findings suggest a predominant Th-2-like response in RSV-infected infants, which could explain some aspects of the immunopathogenesis of RSV infection and the RSV-specific and nonspecific IgE antibody responses observed.

Original languageEnglish (US)
Pages (from-to)190-195
Number of pages6
JournalAmerican Journal of Respiratory and Critical Care Medicine
Volume156
Issue number1
StatePublished - 1997
Externally publishedYes

Fingerprint

Respiratory Syncytial Virus Infections
Respiratory Syncytial Viruses
Interleukin-4
Interferons
Immunoglobulin E
T-Lymphocytes
Pokeweed Mitogens
Virus Diseases
Cellular Immunity
Respiratory Tract Infections
Antibody Formation
Blood Cells
B-Lymphocytes
Lymphocytes
Cytokines
Viruses
Phenotype

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Román, M., Calhoun, W., Hinton, K. L., Avendaño, L. F., Simon, V., Escobar, A. M., ... Díaz, P. V. (1997). Respiratory syncytial virus infection in infants is associated with predominant Th-2-1ike response. American Journal of Respiratory and Critical Care Medicine, 156(1), 190-195.

Respiratory syncytial virus infection in infants is associated with predominant Th-2-1ike response. / Román, Mariana; Calhoun, William; Hinton, Kim L.; Avendaño, Luis F.; Simon, Valeska; Escobar, Ana M.; Gaggero, Aldo; Díaz, Patricia V.

In: American Journal of Respiratory and Critical Care Medicine, Vol. 156, No. 1, 1997, p. 190-195.

Research output: Contribution to journalArticle

Román, M, Calhoun, W, Hinton, KL, Avendaño, LF, Simon, V, Escobar, AM, Gaggero, A & Díaz, PV 1997, 'Respiratory syncytial virus infection in infants is associated with predominant Th-2-1ike response', American Journal of Respiratory and Critical Care Medicine, vol. 156, no. 1, pp. 190-195.
Román, Mariana ; Calhoun, William ; Hinton, Kim L. ; Avendaño, Luis F. ; Simon, Valeska ; Escobar, Ana M. ; Gaggero, Aldo ; Díaz, Patricia V. / Respiratory syncytial virus infection in infants is associated with predominant Th-2-1ike response. In: American Journal of Respiratory and Critical Care Medicine. 1997 ; Vol. 156, No. 1. pp. 190-195.
@article{57e7d74d8bd946dba52327803d17468d,
title = "Respiratory syncytial virus infection in infants is associated with predominant Th-2-1ike response",
abstract = "Viral infections have been associated with cellular immune responses and production of Th-1 cytokines. Respiratory syncytial virus (RSV), however, induces virus-specific IgE, which might be a consequence of a Th-2-like activation. To test this hypothesis we quantified interferon-γ (IFN-γ) and interleukin-4 (IL-4) in the supernatant of peripheral blood mononuclear cells cultured for 24 and 48 h in the presence or absence of phytohemaglutinin and pokeweed mitogen and the lymphocyte phenotypes to analyze subsets and their activation markers, from 15 hospitalized infants during an acute lower respiratory infection caused by RSV and 17 healthy control infants from 1 to 15 mo of age. Compared with the control infants, those infected with RSV had an increase in the number of B-cells (p < 0.02) and decreases in both CD8+ T- cells (p < 0.01) and activated CD8+/CD25+ suppressor/cytotoxic T-cells (p < 0.007). In RSV-infected infants, IFN-γ production was subtotally suppressed, whereas IL-4 production was decreased to a lesser degree, giving significantly (p < 0.001) increased IL-4/IFN-γ ratio compared with that in the control infants. These findings suggest a predominant Th-2-like response in RSV-infected infants, which could explain some aspects of the immunopathogenesis of RSV infection and the RSV-specific and nonspecific IgE antibody responses observed.",
author = "Mariana Rom{\'a}n and William Calhoun and Hinton, {Kim L.} and Avenda{\~n}o, {Luis F.} and Valeska Simon and Escobar, {Ana M.} and Aldo Gaggero and D{\'i}az, {Patricia V.}",
year = "1997",
language = "English (US)",
volume = "156",
pages = "190--195",
journal = "American Journal of Respiratory and Critical Care Medicine",
issn = "1073-449X",
publisher = "American Thoracic Society",
number = "1",

}

TY - JOUR

T1 - Respiratory syncytial virus infection in infants is associated with predominant Th-2-1ike response

AU - Román, Mariana

AU - Calhoun, William

AU - Hinton, Kim L.

AU - Avendaño, Luis F.

AU - Simon, Valeska

AU - Escobar, Ana M.

AU - Gaggero, Aldo

AU - Díaz, Patricia V.

PY - 1997

Y1 - 1997

N2 - Viral infections have been associated with cellular immune responses and production of Th-1 cytokines. Respiratory syncytial virus (RSV), however, induces virus-specific IgE, which might be a consequence of a Th-2-like activation. To test this hypothesis we quantified interferon-γ (IFN-γ) and interleukin-4 (IL-4) in the supernatant of peripheral blood mononuclear cells cultured for 24 and 48 h in the presence or absence of phytohemaglutinin and pokeweed mitogen and the lymphocyte phenotypes to analyze subsets and their activation markers, from 15 hospitalized infants during an acute lower respiratory infection caused by RSV and 17 healthy control infants from 1 to 15 mo of age. Compared with the control infants, those infected with RSV had an increase in the number of B-cells (p < 0.02) and decreases in both CD8+ T- cells (p < 0.01) and activated CD8+/CD25+ suppressor/cytotoxic T-cells (p < 0.007). In RSV-infected infants, IFN-γ production was subtotally suppressed, whereas IL-4 production was decreased to a lesser degree, giving significantly (p < 0.001) increased IL-4/IFN-γ ratio compared with that in the control infants. These findings suggest a predominant Th-2-like response in RSV-infected infants, which could explain some aspects of the immunopathogenesis of RSV infection and the RSV-specific and nonspecific IgE antibody responses observed.

AB - Viral infections have been associated with cellular immune responses and production of Th-1 cytokines. Respiratory syncytial virus (RSV), however, induces virus-specific IgE, which might be a consequence of a Th-2-like activation. To test this hypothesis we quantified interferon-γ (IFN-γ) and interleukin-4 (IL-4) in the supernatant of peripheral blood mononuclear cells cultured for 24 and 48 h in the presence or absence of phytohemaglutinin and pokeweed mitogen and the lymphocyte phenotypes to analyze subsets and their activation markers, from 15 hospitalized infants during an acute lower respiratory infection caused by RSV and 17 healthy control infants from 1 to 15 mo of age. Compared with the control infants, those infected with RSV had an increase in the number of B-cells (p < 0.02) and decreases in both CD8+ T- cells (p < 0.01) and activated CD8+/CD25+ suppressor/cytotoxic T-cells (p < 0.007). In RSV-infected infants, IFN-γ production was subtotally suppressed, whereas IL-4 production was decreased to a lesser degree, giving significantly (p < 0.001) increased IL-4/IFN-γ ratio compared with that in the control infants. These findings suggest a predominant Th-2-like response in RSV-infected infants, which could explain some aspects of the immunopathogenesis of RSV infection and the RSV-specific and nonspecific IgE antibody responses observed.

UR - http://www.scopus.com/inward/record.url?scp=0030831247&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030831247&partnerID=8YFLogxK

M3 - Article

C2 - 9230746

AN - SCOPUS:0030831247

VL - 156

SP - 190

EP - 195

JO - American Journal of Respiratory and Critical Care Medicine

JF - American Journal of Respiratory and Critical Care Medicine

SN - 1073-449X

IS - 1

ER -