Responses in the aged rat brain after total immunolesion

Zezong Gu, Juan Yu, J. Regino Perez-Polo

    Research output: Contribution to journalArticle

    30 Citations (Scopus)

    Abstract

    In the present study, we compare the effects of cholinergic deafferentation of the hippocampus, cortex, and olfactory bulb of young and aged rats on nerve growth factor (NGF) protein levels in these areas. We also describe glial responses to intraventricular injections of the immunotoxin, 192 IgG-saporin in the aged. Choline acetyltransferase (ChAT) activity was dramatically decreased in the basal forebrain and target areas of the cholinergic basal forebrain neurons (CBFNs) in the young immunolesioned rats and to a lesser extent in their aged counterparts. After total immunolesion, NGF protein levels significantly increased in the hippocampus, cortex, and olfactory bulb of the young rats but not of the aged rats, except for small increases in the olfactory bulb after two weeks. After immunolesion NGF protein levels in the basal forebrain increased in young rats and less so in the aged rats. The total immunolesions had no effects on NGF and BDNF mRNA levels in the hippocampus and cortex. Two weeks after injection of the immunotoxin, the profiles of ACHE- and p75(NTR)-positive cells significantly decreased in medial septum, vertical and horizontal limbs of diagonal band and nucleus basalis of Meynert. There was also an increase in microglia while but not astrocytes in the subnuclei of basal forebrain. In conclusion, 192 IgG-saporin was effective in producing cholinergic lesions in both young and aged rat brains, the lesion-induced NGF response was partially extinguished in the aged rat brains and immunolesions induced a microglial response in aged brain.

    Original languageEnglish (US)
    Pages (from-to)7-16
    Number of pages10
    JournalJournal of Neuroscience Research
    Volume54
    Issue number1
    DOIs
    StatePublished - Oct 1 1998

    Fingerprint

    Nerve Growth Factor
    Brain
    Olfactory Bulb
    Cholinergic Agents
    Immunotoxins
    Hippocampus
    Basal Nucleus of Meynert
    Intraventricular Injections
    Proteins
    Choline O-Acetyltransferase
    Brain-Derived Neurotrophic Factor
    Microglia
    Neuroglia
    Astrocytes
    Extremities
    Neurons
    Messenger RNA
    Injections
    Basal Forebrain
    192 IgG-saporin

    Keywords

    • Aging
    • ChAT
    • Cholinergic basal forebrain neurons
    • ELISA
    • Immunolesion
    • mRNA
    • NGF

    ASJC Scopus subject areas

    • Neuroscience(all)

    Cite this

    Responses in the aged rat brain after total immunolesion. / Gu, Zezong; Yu, Juan; Perez-Polo, J. Regino.

    In: Journal of Neuroscience Research, Vol. 54, No. 1, 01.10.1998, p. 7-16.

    Research output: Contribution to journalArticle

    Gu, Zezong ; Yu, Juan ; Perez-Polo, J. Regino. / Responses in the aged rat brain after total immunolesion. In: Journal of Neuroscience Research. 1998 ; Vol. 54, No. 1. pp. 7-16.
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