Abstract
The responses to endothelial vasodilators and exogenous nitric oxide (NO) were characterized in intact isolated uterine vascular beds of nonpregnant, midpregnant and late-pregnant rats perfused with Kreb's buffer (37°C, 5% CO2 in air, pH ∼7.4) containing 2% dextran and indomethacin. Phenylephrine increased perfusion pressure in the vascular beds equally in all three groups. In the presence of phenylephrine, Nω-nitro-L-arginine methyl ester (L-NAME) significantly augmented perfusion pressure in the order: nonpregnant<midpregnant<late-pregnant uterine vascular bed. Acetylcholine and bradykinin-induced attenuation of perfusion pressure did not depend on gestational age. The decrease in perfusion pressure induced by acetylcholine was nonsignificantly attenuated by L-NAME in vascular beds from pregnant rats. The attenuation induced by bradykinin reached significant level in the vascular beds from midpregnant rats. The diethylamine (DEA)/NO-induced decrease in perfusion pressure was not influenced by L-NAME in any group. These data demonstrate the augmentation of basal release of NO associated with progression of pregnancy, while the responses to endothelial vasodilators do not depend on gestational age and are not abolished by inhibition of NO synthase, suggesting involvement of nonprostanoid non-NO factor in the control of uterine circulation.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 297-301 |
| Number of pages | 5 |
| Journal | General Pharmacology: Vascular System |
| Volume | 35 |
| Issue number | 6 |
| DOIs | |
| State | Published - 2000 |
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Keywords
- EDHF
- EDRF
- Endothelium
- Smooth muscle
- Uterine circulation
ASJC Scopus subject areas
- Pharmacology
Cite this
Responses of isolated perfused uterine vascular beds of nonpregnant and pregnant rats to endogenous and exogenous nitric oxide. / Fulep, E. E.; Vedernikov, Y. P.; Saade, George; Garfield, R. E.
In: General Pharmacology: Vascular System, Vol. 35, No. 6, 2000, p. 297-301.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Responses of isolated perfused uterine vascular beds of nonpregnant and pregnant rats to endogenous and exogenous nitric oxide
AU - Fulep, E. E.
AU - Vedernikov, Y. P.
AU - Saade, George
AU - Garfield, R. E.
PY - 2000
Y1 - 2000
N2 - The responses to endothelial vasodilators and exogenous nitric oxide (NO) were characterized in intact isolated uterine vascular beds of nonpregnant, midpregnant and late-pregnant rats perfused with Kreb's buffer (37°C, 5% CO2 in air, pH ∼7.4) containing 2% dextran and indomethacin. Phenylephrine increased perfusion pressure in the vascular beds equally in all three groups. In the presence of phenylephrine, Nω-nitro-L-arginine methyl ester (L-NAME) significantly augmented perfusion pressure in the order: nonpregnant
AB - The responses to endothelial vasodilators and exogenous nitric oxide (NO) were characterized in intact isolated uterine vascular beds of nonpregnant, midpregnant and late-pregnant rats perfused with Kreb's buffer (37°C, 5% CO2 in air, pH ∼7.4) containing 2% dextran and indomethacin. Phenylephrine increased perfusion pressure in the vascular beds equally in all three groups. In the presence of phenylephrine, Nω-nitro-L-arginine methyl ester (L-NAME) significantly augmented perfusion pressure in the order: nonpregnant
KW - EDHF
KW - EDRF
KW - Endothelium
KW - Smooth muscle
KW - Uterine circulation
UR - http://www.scopus.com/inward/record.url?scp=1842831885&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=1842831885&partnerID=8YFLogxK
U2 - 10.1016/S0306-3623(02)00114-3
DO - 10.1016/S0306-3623(02)00114-3
M3 - Article
C2 - 11922959
AN - SCOPUS:1842831885
VL - 35
SP - 297
EP - 301
JO - Vascular Pharmacology
JF - Vascular Pharmacology
SN - 1537-1891
IS - 6
ER -