Resposta imunológica e bioquímica de bezerros imunizados com rMSP1a (Anaplasma marginale)utilizando nanotubos de carbono como moléculas carreadoras

Translated title of the contribution: Immune response and biochemistry of calves immunized with rMSP1a (Anaplasma marginale) using carbon nanotubes as carrier molecules

Bruna Torres Silvestre, Júlia Angélica Gonçalves Da Silveira, Elias Jorge Facury-Filho, Antônio Último De Carvalho, Alice Freitas Versiani, Letícia Gracielle Tôrres de Miranda Estevam, Márcio Sobreira Silva Araújo, Olindo Assis Martins-Filho, Deborah Aparecida Negrão-Corrêa, Múcio Flávio Barbosa Ribeiro

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Vaccination against Anaplasma marginale has been considered an important control strategy for bovine anaplasmosis. Recently, mice immunized with rMSP1a linked to carbon nanotubes (MWNT) showed significant immune responses, generating a new possibility for use of an inactivated vaccine. The objective of this study was to investigate the cellular and humoral responses in calves immunized with MWNT+rMSP1a, associated with inactivated vaccine of A. marginale produced in vitro, and evaluate the toxic effects of the MWNT on renal and hepatic function. rMSP1a was covalently linked to MWNT. Inactivated vaccine (AmUFMG2) was produced by cultivating A. marginale in IDE8 cells. Twenty four Holstein calves were divided (four groups) and immunized subcutaneously with PBS and non-carboxylated MWNT (control, G1), AmUFMG2 (G2), MWNT+rMSP1a (G3), and AmUFMG2 with MWNT+rMSP1a (G4). Blood samples were collected for total leukocyte counts, biochemical profiling and evaluation of the cellular and humoral response. Immunization with MWNT+rMSP1a induced increase in the total number of leukocytes, NK cells, in the lymphocyte populations and higher levels of antibodies compared to calves immunized only with AmUFMG2. Furthermore, MWNT did not induce changes in the biochemical profile. These data indicate that MWNT+rMSP1a were able to induce the immune responses more efficiently than AmUFMG2 alone, without generating toxicity..

Translated title of the contributionImmune response and biochemistry of calves immunized with rMSP1a (Anaplasma marginale) using carbon nanotubes as carrier molecules
Original languagePortuguese
Pages (from-to)191-202
Number of pages12
JournalRevista Brasileira de Parasitologia Veterinaria
Volume27
Issue number2
DOIs
StatePublished - Apr 1 2018
Externally publishedYes

Keywords

  • Anaplasma marginale
  • Carbon nanotubes
  • Inactivate vaccine
  • MSP1a

ASJC Scopus subject areas

  • Parasitology
  • veterinary(all)

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