Restoration of immunity in burned mice by cimetidine

Edward M. Bender, John F. Hansbrough, Ramon Zapata Sirvent, John Sullivan, Henry N. Claman

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

We have previously described a mouse model of postburn suppression of cell-mediated immunity (CMI). Ear swelling (ES) in response to contact antigen challenge is depressed maximally 14 days following a 25% steam burn and recovers to control levels 3 weeks postburn. Splenic lymphocyte proliferation in response to Concanavalin A (Con A) is also depressed 14 days postburn. Splenic T-lymphocyte subset analysis with monoclonal antibodies for helper cells (Lyt 1.2) and suppressor cells (Lyt 2.2) reveals that T-helper cells reach a minimal level and T suppressor cells reach a maximum level 14 days postburn. The helper: Suppressor ratio (HSR) reaches its nadir at day 14. Treatment of burned mice with low-dose cimetidine (2 or 10 mg/kg/day), but not high-dose (50 mg/kg/day), for 14 days restores CMI. Low-dose cimetidine also normalizes the HSR but does not effect postburn depression of mitogen responsiveness. Low-dose cimetidine probably restores CMI by inhibiting suppressor cells, whereas high doses provide more global inhibition. Recovery of mitogen responsiveness may require continued cimetidine presence in culture.

Original languageEnglish (US)
Pages (from-to)131-137
Number of pages7
JournalJournal of Trauma - Injury, Infection and Critical Care
Volume25
Issue number2
StatePublished - Jan 1 1985
Externally publishedYes

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine
  • Surgery

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    Bender, E. M., Hansbrough, J. F., Zapata Sirvent, R., Sullivan, J., & Claman, H. N. (1985). Restoration of immunity in burned mice by cimetidine. Journal of Trauma - Injury, Infection and Critical Care, 25(2), 131-137.