Restriction enzyme analysis of American region dengue viruses

V. Vorndam, R. M R Nogueira, D. W. Trent

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Restriction fragment heterogeneity of Hae III digestion products of cDNA to virion RNA was used to map the distribution of dengue virus topotypes found in the American region. By comparing the electrophoretic patterns of fragments produced, dengue virus isolates were placed in groups that agreed with those previously determined by oligonucleotide fingerprinting. Dengue-1 and dengue-4 viruses occur throughout the western hemisphere as single genetic types, with most of the isolates sharing at least 70% of their Hae III restriction enzyme fragments. Dengue-2 virus exists as two topotypes in the region with apparently non-overlapping distributions. The Puerto Rico topotype, which has been in the Caribbean for at least 40 years, is genetically diverse, while the Jamaica topotype, first isolated in 1981, is more homogeneous and has expanded its range from the original Caribbean focus to South America.

Original languageEnglish (US)
Pages (from-to)191-196
Number of pages6
JournalArchives of Virology
Volume136
Issue number1-2
DOIs
StatePublished - Mar 1994

Fingerprint

Restriction Mapping
Dengue Virus
Jamaica
Puerto Rico
Dengue
South America
Oligonucleotides
Virion
Digestion
Complementary DNA
RNA
Enzymes

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Genetics

Cite this

Vorndam, V., Nogueira, R. M. R., & Trent, D. W. (1994). Restriction enzyme analysis of American region dengue viruses. Archives of Virology, 136(1-2), 191-196. https://doi.org/10.1007/BF01538828

Restriction enzyme analysis of American region dengue viruses. / Vorndam, V.; Nogueira, R. M R; Trent, D. W.

In: Archives of Virology, Vol. 136, No. 1-2, 03.1994, p. 191-196.

Research output: Contribution to journalArticle

Vorndam, V, Nogueira, RMR & Trent, DW 1994, 'Restriction enzyme analysis of American region dengue viruses', Archives of Virology, vol. 136, no. 1-2, pp. 191-196. https://doi.org/10.1007/BF01538828
Vorndam, V. ; Nogueira, R. M R ; Trent, D. W. / Restriction enzyme analysis of American region dengue viruses. In: Archives of Virology. 1994 ; Vol. 136, No. 1-2. pp. 191-196.
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