Retinal non-visual photoreception in space

Susana B. Zanello, Audrey Nguyen, Corey A. Theriot

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background: Circadian rhythm disruption occurs during spaceflight, leading to crew health and performance decrements. Spaceflight-related retinal changes, including oxidative stress and neuronal loss, have been previously reported in mice. Methods: Animal tissue from experiments aboard shuttle missions STS-133 (BALB/cJ mice, albino strain) and STS-135 (C57BL mice, pigmented strain), along with ground controls, was examined to determine survival of intrinsically photosensitive retinal ganglion cells (iPRGC) and melanopsin expression in retinas of mice exposed to the spaceflight environment. Real-time qPCR (RTqPCT) and microarray approaches were used to analyze Opn4 (melanopsin) gene expression, while immunohistologic studies were conducted to detect melanopsin localization in the retina. Results: Opn4 expression was decreased in albino BALB/cJ mice exposed to spaceflight, as measured by RTqPCR, but not in C57BL mice samples as analyzed by microarray. Opn4 expression returned to control levels at 7 d postreturn in the BALB/cJ samples. Melanopsin positive RGCs were found in the expected proportion in all samples, except for the BALB/cJ samples at 1 d after flight, where virtually no immunoreactive cells were found. Discussion: Spaceflight environmental factors may affect the nonvisual function of the retina, mediated by a reduction in melanopsin expression and ipRGC survival, contributing to circadian disruption.

Original languageEnglish (US)
Pages (from-to)1277-1280
Number of pages4
JournalAviation Space and Environmental Medicine
Volume84
Issue number12
DOIs
StatePublished - Dec 2013

Fingerprint

Space Flight
Retina
Inbred C57BL Mouse
Retinal Ganglion Cells
Circadian Rhythm
Oxidative Stress
melanopsin
Gene Expression
Health

Keywords

  • Circadian rhythm
  • Melanopsin
  • Opsin 4
  • Retina
  • Spaceflight

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health

Cite this

Retinal non-visual photoreception in space. / Zanello, Susana B.; Nguyen, Audrey; Theriot, Corey A.

In: Aviation Space and Environmental Medicine, Vol. 84, No. 12, 12.2013, p. 1277-1280.

Research output: Contribution to journalArticle

Zanello, SB, Nguyen, A & Theriot, CA 2013, 'Retinal non-visual photoreception in space', Aviation Space and Environmental Medicine, vol. 84, no. 12, pp. 1277-1280. https://doi.org/10.3357/ASEM.3762.2013
Zanello, Susana B. ; Nguyen, Audrey ; Theriot, Corey A. / Retinal non-visual photoreception in space. In: Aviation Space and Environmental Medicine. 2013 ; Vol. 84, No. 12. pp. 1277-1280.
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