Retinoic acid induces apoptosis in PC12 cells independent of neurotrophic factors

Liqi Tong, Karin Werrbach-Perez, J. Regino Perez-Polo

    Research output: Contribution to journalArticle

    22 Citations (Scopus)

    Abstract

    PC12 cells are known to undergo programmed cell death (apoptosis) when they are deprived of serum. Nerve growth factor (NGF) rescues PC12 cells from serum deprivation-induced apoptosis. In the present study, we examined the effects of retinoic acid (RA), a classic morphogen, on apoptosis in PC12 cells after serum deprivation and NGF-mediated rescue. In naive PC12 cells, all trans-RA treatment induced cell death in the presence of NGF. RA also abolished the protective effects of dibutyryl cyclic AMP or insulin under serum-free conditions. The death process was accompanied by nuclear condensation and DNA fragmentation, typical of apoptosis. In addition, RA also increased the extent of apoptosis in PC12 cells after serum deprivation. Cycloheximide, an inhibitor of protein synthesis, did not abolish the effects of RA on serum-deprived PC12 cells. RA also decreased thymidine incorporation and proliferation in NGF-treated PC12 cells. Furthermore, although the total DNA binding activity of the AP-1 transcription factor was not changed after RA treatment, RA decreased a specific AP-1 transcriptional activity. It is surprising that differentiated PC12 cells resisted the toxic effects of RA. These data suggest that RA might function as an endogenous inducer of apoptosis during neural differentiation by a mechanism distinct from that of serum deprivation.

    Original languageEnglish (US)
    Pages (from-to)1424-1435
    Number of pages12
    JournalJournal of Neurochemistry
    Volume68
    Issue number4
    StatePublished - Apr 1997

    Fingerprint

    PC12 Cells
    Nerve Growth Factors
    Tretinoin
    Apoptosis
    Nerve Growth Factor
    Serum
    Transcription Factor AP-1
    Cell death
    Cell Death
    Bucladesine
    Protein Synthesis Inhibitors
    Poisons
    DNA
    DNA Fragmentation
    Cycloheximide
    Thymidine
    Condensation
    Insulin

    Keywords

    • AP-1
    • Apoptosis
    • Nerve growth factor
    • PC12
    • Retinoic acid
    • Serum deprivation

    ASJC Scopus subject areas

    • Biochemistry
    • Cellular and Molecular Neuroscience

    Cite this

    Tong, L., Werrbach-Perez, K., & Perez-Polo, J. R. (1997). Retinoic acid induces apoptosis in PC12 cells independent of neurotrophic factors. Journal of Neurochemistry, 68(4), 1424-1435.

    Retinoic acid induces apoptosis in PC12 cells independent of neurotrophic factors. / Tong, Liqi; Werrbach-Perez, Karin; Perez-Polo, J. Regino.

    In: Journal of Neurochemistry, Vol. 68, No. 4, 04.1997, p. 1424-1435.

    Research output: Contribution to journalArticle

    Tong, L, Werrbach-Perez, K & Perez-Polo, JR 1997, 'Retinoic acid induces apoptosis in PC12 cells independent of neurotrophic factors', Journal of Neurochemistry, vol. 68, no. 4, pp. 1424-1435.
    Tong, Liqi ; Werrbach-Perez, Karin ; Perez-Polo, J. Regino. / Retinoic acid induces apoptosis in PC12 cells independent of neurotrophic factors. In: Journal of Neurochemistry. 1997 ; Vol. 68, No. 4. pp. 1424-1435.
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