Retinoic acid inhibition of serum-induced c-fos transcription in a fibrosarcoma cell line

Pamela Jaffey, Lee Nien L Chan, Jinyi Shao, Jürgen Schneider-Schaulies, Teh Sheng Chan

Research output: Contribution to journalArticle

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Abstract

We investigated the mechanism by which retinoic acid causes growth arrest and flat reversion of SSV-NRK, simian sarcoma virus-transformed normal rat kidney cells. Northern analysis revealed that both chronic (7 days) and acute (6 h) retinoic acid treatment of serum-stimulated SSVNRK cells caused a 6-fold decrease in c-fos mRNA levels. In addition, nuclear run-on experiments showed that retinoic acid regulated c-fos expression in SSV-NRK cells at the transcriptional initiation level. Attenuation of c-fos transcription was equal in both retinoic acid-treated and control cells, and no increased c-fos mRNA turnover was detected in retinoic acid-treated cells. Furthermore, there was no observed change in the c-fos mRNA levels after only 30 min of retinoic acid treatment, suggesting that a mechanism involving the interruption of the signal transduction mechanism at the membrane level is unlikely. Because it has been shown that c-fos expression plays a pivotal role in mitogenesis of quiescent fibroblasts, we conclude that the retinoic acid-mediated down-regulation of c-fos expression is a mechanism for growth inhibition in SSV-NRK cells.

Original languageEnglish (US)
Pages (from-to)2384-2388
Number of pages5
JournalCancer Research
Volume52
Issue number9
StatePublished - May 1 1992

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Fibrosarcoma
Tretinoin
Cell Line
Serum
Messenger RNA
Woolly Monkey Sarcoma Virus
Growth
Signal Transduction
Down-Regulation
Fibroblasts
Kidney
Membranes

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Jaffey, P., Chan, L. N. L., Shao, J., Schneider-Schaulies, J., & Chan, T. S. (1992). Retinoic acid inhibition of serum-induced c-fos transcription in a fibrosarcoma cell line. Cancer Research, 52(9), 2384-2388.

Retinoic acid inhibition of serum-induced c-fos transcription in a fibrosarcoma cell line. / Jaffey, Pamela; Chan, Lee Nien L; Shao, Jinyi; Schneider-Schaulies, Jürgen; Chan, Teh Sheng.

In: Cancer Research, Vol. 52, No. 9, 01.05.1992, p. 2384-2388.

Research output: Contribution to journalArticle

Jaffey, P, Chan, LNL, Shao, J, Schneider-Schaulies, J & Chan, TS 1992, 'Retinoic acid inhibition of serum-induced c-fos transcription in a fibrosarcoma cell line', Cancer Research, vol. 52, no. 9, pp. 2384-2388.
Jaffey P, Chan LNL, Shao J, Schneider-Schaulies J, Chan TS. Retinoic acid inhibition of serum-induced c-fos transcription in a fibrosarcoma cell line. Cancer Research. 1992 May 1;52(9):2384-2388.
Jaffey, Pamela ; Chan, Lee Nien L ; Shao, Jinyi ; Schneider-Schaulies, Jürgen ; Chan, Teh Sheng. / Retinoic acid inhibition of serum-induced c-fos transcription in a fibrosarcoma cell line. In: Cancer Research. 1992 ; Vol. 52, No. 9. pp. 2384-2388.
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