Retrospective serological and genetic study of the distribution of hantaviruses in Greece

A. Papa, A. M. Johnson, P. C. Stockton, M. D. Bowen, C. F. Spiropoulou, S. Alexiou-Daniel, Thomas Ksiazek, S. T. Nichol, A. Antoniadis

Research output: Contribution to journalArticle

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Abstract

A retrospective serological and genetic study of hantaviruses responsible for hemorrhagic fever with renal syndrome (HFRS) in Greece during the last 17 years is presented. Fifty-one serum samples taken from 30 HFRS cases previously diagnosed by immunofluorescence assay were tested by ELISA for IgG (Hantaan, Dobrava, and Puumala) and IgM antibodies (Hantaan and Puumala). Results were compatible with the majority of infections being related to hantaviruses carried by rodents of the subfamily Murinae. RNA was extracted from 26 selected samples and reverse transcriptase-polymerase chain reaction (RT-PCR) was performed using primers specifically designed for the detection of hantaviruses associated with murine (MS-N-specific, MM-G1-specific primers) or arvicoline rodents (PPT-N-specific primers). In addition, primers previously designed for the detection of the G2 coding region of the Murinae-associated hantaviruses were also used. Sequencing of the PCR products was then performed, followed by phylogenetic analysis of nucleotide sequence differences. Eleven out of the 26 serum samples tested were found to be positive by PCR with the MS-N primers, whereas four were positive with the MM-G1 primers, and only two with the G2 primers. None of the samples was found positive with the PPT primers. The sequence analysis showed that the virus that was responsible for these 11 HFRS cases was the Dobrava virus, which is endemic throughout the Balkans.

Original languageEnglish (US)
Pages (from-to)321-327
Number of pages7
JournalJournal of Medical Virology
Volume55
Issue number4
DOIs
StatePublished - Aug 1998
Externally publishedYes

Fingerprint

Hantavirus
Greece
Hemorrhagic Fever with Renal Syndrome
Murinae
Rodentia
Balkan Peninsula
Viruses
Polymerase Chain Reaction
Arvicolinae
Reverse Transcriptase Polymerase Chain Reaction
Serum
Fluorescent Antibody Technique
Immunoglobulin M
Sequence Analysis
Immunoglobulin G
Enzyme-Linked Immunosorbent Assay
RNA
Antibodies
Infection

Keywords

  • ELISA
  • Hantavirus
  • Hemorrhagic fever with renal syndrome
  • RT-PCR

ASJC Scopus subject areas

  • Virology

Cite this

Papa, A., Johnson, A. M., Stockton, P. C., Bowen, M. D., Spiropoulou, C. F., Alexiou-Daniel, S., ... Antoniadis, A. (1998). Retrospective serological and genetic study of the distribution of hantaviruses in Greece. Journal of Medical Virology, 55(4), 321-327. https://doi.org/10.1002/(SICI)1096-9071(199808)55:4<321::AID-JMV11>3.0.CO;2-H

Retrospective serological and genetic study of the distribution of hantaviruses in Greece. / Papa, A.; Johnson, A. M.; Stockton, P. C.; Bowen, M. D.; Spiropoulou, C. F.; Alexiou-Daniel, S.; Ksiazek, Thomas; Nichol, S. T.; Antoniadis, A.

In: Journal of Medical Virology, Vol. 55, No. 4, 08.1998, p. 321-327.

Research output: Contribution to journalArticle

Papa, A, Johnson, AM, Stockton, PC, Bowen, MD, Spiropoulou, CF, Alexiou-Daniel, S, Ksiazek, T, Nichol, ST & Antoniadis, A 1998, 'Retrospective serological and genetic study of the distribution of hantaviruses in Greece', Journal of Medical Virology, vol. 55, no. 4, pp. 321-327. https://doi.org/10.1002/(SICI)1096-9071(199808)55:4<321::AID-JMV11>3.0.CO;2-H
Papa, A. ; Johnson, A. M. ; Stockton, P. C. ; Bowen, M. D. ; Spiropoulou, C. F. ; Alexiou-Daniel, S. ; Ksiazek, Thomas ; Nichol, S. T. ; Antoniadis, A. / Retrospective serological and genetic study of the distribution of hantaviruses in Greece. In: Journal of Medical Virology. 1998 ; Vol. 55, No. 4. pp. 321-327.
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