Revascularization and remodelling of pancreatic islets grafted under the kidney capsule

Sergio Morini, Melissa L. Brown, Luca Cicalese, George Elias, Simone Carotti, Eugenio Gaudio, Cristiana Rastellini

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

The revascularization and the structural changes resulting from interactions between the graft and the host were investigated in transplanted pancreatic islets under the kidney capsule. Islets were isolated from mice pancreata and transplanted in syngeneic diabetic animals. Graft-bearing kidneys were collected on different days post-transplant and processed for light microscopy, immunohistochemistry and transmission electron microscopy. A numerical analysis was performed in order to compare the percentage number of the different types of cells in native islets and at different time points after the transplant. Recipient animals reversed diabetes within 4 days. An intraperitoneal glucose tolerance test was performed to determine islet functionality under stressful conditions. During the initial few days post-transplant, the islets showed peculiar shapes and the graft tended to aggregate along the vessels. Starting at days 4-7 post-transplant, islets were revascularized from vessels connected to both the cortical and the capsular vascular network of the kidney. From day 7-14 post-transplant, the vessels progressively appeared more similar in features and size to those of in situ pancreatic islets. Both the percentage number of the different cell types and the distribution of Alpha, Beta and Delta cells inside the graft were significantly different as compared with intact islets, demonstrating quantitative and structural changes after the engraftment. No concomitant proliferation of Beta cells was detected using a bromodeoxyuridin staining method. Despite the fact that quick revascularization preserved a large mass of tissue, the remodelling process of the graft and the newly formed vascularization led to a different organization of the endocrine tissue as compared with intact in situ islets. This constitutes the morphological basis for alterations of the normal intercellular interactions and may explain the altered secretory cell function often observed in transplant.

Original languageEnglish (US)
Pages (from-to)565-577
Number of pages13
JournalJournal of Anatomy
Volume210
Issue number5
DOIs
StatePublished - May 2007
Externally publishedYes

Fingerprint

islets of Langerhans
Islets of Langerhans
Capsules
vessel
kidneys
Transplants
Kidney
structural change
diabetes
animal
cells
transmission electron microscopy
microscopy
glucose
tolerance
cell aggregates
blood vessels
pancreas
immunohistochemistry
light microscopy

Keywords

  • Islets of Langerhans
  • Revascularization
  • Structure
  • Transplantation
  • Ultrastructure

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Anatomy

Cite this

Revascularization and remodelling of pancreatic islets grafted under the kidney capsule. / Morini, Sergio; Brown, Melissa L.; Cicalese, Luca; Elias, George; Carotti, Simone; Gaudio, Eugenio; Rastellini, Cristiana.

In: Journal of Anatomy, Vol. 210, No. 5, 05.2007, p. 565-577.

Research output: Contribution to journalArticle

Morini, S, Brown, ML, Cicalese, L, Elias, G, Carotti, S, Gaudio, E & Rastellini, C 2007, 'Revascularization and remodelling of pancreatic islets grafted under the kidney capsule', Journal of Anatomy, vol. 210, no. 5, pp. 565-577. https://doi.org/10.1111/j.1469-7580.2007.00717.x
Morini, Sergio ; Brown, Melissa L. ; Cicalese, Luca ; Elias, George ; Carotti, Simone ; Gaudio, Eugenio ; Rastellini, Cristiana. / Revascularization and remodelling of pancreatic islets grafted under the kidney capsule. In: Journal of Anatomy. 2007 ; Vol. 210, No. 5. pp. 565-577.
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