Reverse genetics of Zika virus

Research output: Chapter in Book/Report/Conference proceedingChapter

4 Scopus citations

Abstract

We have established a reverse genetic system for Zika virus (ZIKV). Five shuttle plasmids were constructed and assembled into the full-length cDNA clone of ZIKV genome. To ensure the stability of the cDNA clone, we used a low copy vector (pACYC177) and a set of unique restriction enzyme sites on the ZIKV genome to assemble the full-length cDNA clone. A T7 promoter was engineered in front of the viral 5′ UTR for in vitro transcription. A hepatitis delta virus ribozyme (HDVr) sequence was engineered following the viral 3′ UTR for generation of the authentic 3′ end of the RNA transcript.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages47-58
Number of pages12
Volume1602
DOIs
StatePublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1602
ISSN (Print)1064-3745

Keywords

  • Dengue
  • Flavivirus
  • Guillain-Barré syndrome
  • Microcephaly
  • Mosquito
  • West Nile
  • Zika

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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