@inbook{272b05b970e44db982b4c52ed187781a,
title = "Reverse genetics of Zika virus",
abstract = "We have established a reverse genetic system for Zika virus (ZIKV). Five shuttle plasmids were constructed and assembled into the full-length cDNA clone of ZIKV genome. To ensure the stability of the cDNA clone, we used a low copy vector (pACYC177) and a set of unique restriction enzyme sites on the ZIKV genome to assemble the full-length cDNA clone. A T7 promoter was engineered in front of the viral 5′ UTR for in vitro transcription. A hepatitis delta virus ribozyme (HDVr) sequence was engineered following the viral 3′ UTR for generation of the authentic 3′ end of the RNA transcript.",
keywords = "Dengue, Flavivirus, Guillain-Barr{\'e} syndrome, Microcephaly, Mosquito, West Nile, Zika",
author = "Chao Shan and Xuping Xie and Shi, {Pei Yong}",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media LLC 2017.",
year = "2017",
doi = "10.1007/978-1-4939-6964-7_4",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "47--58",
booktitle = "Methods in Molecular Biology",
}