Reverse genetics of Zika virus

Chao Shan, Xuping Xie, Pei-Yong Shi

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Citations (Scopus)

Abstract

We have established a reverse genetic system for Zika virus (ZIKV). Five shuttle plasmids were constructed and assembled into the full-length cDNA clone of ZIKV genome. To ensure the stability of the cDNA clone, we used a low copy vector (pACYC177) and a set of unique restriction enzyme sites on the ZIKV genome to assemble the full-length cDNA clone. A T7 promoter was engineered in front of the viral 5′ UTR for in vitro transcription. A hepatitis delta virus ribozyme (HDVr) sequence was engineered following the viral 3′ UTR for generation of the authentic 3′ end of the RNA transcript.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages47-58
Number of pages12
Volume1602
DOIs
StatePublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1602
ISSN (Print)1064-3745

Fingerprint

Reverse Genetics
Complementary DNA
Clone Cells
Genome
Hepatitis Delta Virus
Catalytic RNA
5' Untranslated Regions
3' Untranslated Regions
Plasmids
RNA
Enzymes
Zika Virus

Keywords

  • Dengue
  • Flavivirus
  • Guillain-Barré syndrome
  • Microcephaly
  • Mosquito
  • West Nile
  • Zika

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Shan, C., Xie, X., & Shi, P-Y. (2017). Reverse genetics of Zika virus. In Methods in Molecular Biology (Vol. 1602, pp. 47-58). (Methods in Molecular Biology; Vol. 1602). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6964-7_4

Reverse genetics of Zika virus. / Shan, Chao; Xie, Xuping; Shi, Pei-Yong.

Methods in Molecular Biology. Vol. 1602 Humana Press Inc., 2017. p. 47-58 (Methods in Molecular Biology; Vol. 1602).

Research output: Chapter in Book/Report/Conference proceedingChapter

Shan, C, Xie, X & Shi, P-Y 2017, Reverse genetics of Zika virus. in Methods in Molecular Biology. vol. 1602, Methods in Molecular Biology, vol. 1602, Humana Press Inc., pp. 47-58. https://doi.org/10.1007/978-1-4939-6964-7_4
Shan C, Xie X, Shi P-Y. Reverse genetics of Zika virus. In Methods in Molecular Biology. Vol. 1602. Humana Press Inc. 2017. p. 47-58. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-6964-7_4
Shan, Chao ; Xie, Xuping ; Shi, Pei-Yong. / Reverse genetics of Zika virus. Methods in Molecular Biology. Vol. 1602 Humana Press Inc., 2017. pp. 47-58 (Methods in Molecular Biology).
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