Reverse Genetics System for Rift Valley Fever Virus

Breanna Tercero, Shinji Makino

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Rift Valley fever virus (RVFV) is an important mosquito-borne virus that can cause severe disease manifestations in humans including ocular damage, vision loss, late-onset encephalitis, and hemorrhagic fever. In ruminants, RVFV can cause high mortality rates in young animals and high rates of abortion in pregnant animals resulting in an enormous negative impact on the economy of affected regions. To date, no licensed vaccines in humans or anti-RVFV therapeutics for animal or human use are available. The development of reverse genetics has facilitated the generation of recombinant infectious viruses that serve as powerful tools for investigating the molecular biology and pathogenesis of RVFV. Infectious recombinant RVFV can be rescued entirely from cDNAs containing predetermined mutations in their genomes to investigate virus–host interactions and mechanisms of pathogenesis and generate live-attenuated vaccines. In this chapter, we will describe the experimental procedures for the implementation of RVFV reverse genetics.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages101-113
Number of pages13
DOIs
StatePublished - Jan 2024

Publication series

NameMethods in Molecular Biology
Volume2733
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Plaque assay
  • Recombinant Rift Valley fever virus
  • Reverse genetics
  • T7 polymerase-driven rescue system
  • Transfection of cDNAs
  • Virus amplification

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Fingerprint

Dive into the research topics of 'Reverse Genetics System for Rift Valley Fever Virus'. Together they form a unique fingerprint.

Cite this