Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis of nonsentinel nodes following completion lymphadenectomy for melanoma

William R. Wrightson, Sandra L. Wong, Michael J. Edwards, Celia Chao, Andrew J. Conrad, Jeffrey Albrecht, Vicki Viar, Kelly M. McMasters

Research output: Contribution to journalArticle

24 Scopus citations


Introduction: Most melanoma patients with sentinel lymph nodes (SLN) that are histologically positive for metastasis have no additional positive lymph nodes found upon completion lymph node dissection (CLND). Therefore, it has been suggested that CLND may not be required for all patients with positive SLN. This study was undertaken to determine the frequency with which nonsentinel nodes contain melanoma cells detected by RT-PCR. Methods: Negative control lymph nodes were obtained from patients with breast and colon cancer. Positive control lymph nodes contained histologic evidence of melanoma. Nonsentinel nodes were harvested from melanoma patients undergoing CLND for a positive SLN. RT-PCR analysis for melanoma markers tyrosinase, gp100, MART-1, and MAGE-3 was performed, with Southern blot detection. The RT-PCR test was considered positive for the presence of melanoma cells if tyrosinase and at least one other marker were detected above background levels. Results: RT-PCR analysis detected the presence of melanoma cells in 0/100 (0%) of negative control lymph nodes and 28/29 (97%) of positive control lymph nodes. A total of 117 histologically negative nonsentinel nodes from 13 patients who underwent CLND for positive SLN were evaluated. RT-PCR analysis was positive in 18/117 histologically negative nonsentinel nodes (15%) from 7113 patients (54%). Conclusion: RT-PCR analysis suggests that when the SLN contains histologic evidence of melanoma, the remaining nodes in that basin are at risk for metastatic disease, despite the fact that these nonsentinel nodes are infrequently histologically positive.

Original languageEnglish (US)
Pages (from-to)47-51
Number of pages5
JournalJournal of Surgical Research
Issue number1
StatePublished - Jun 1 2001
Externally publishedYes



  • Lymphadenectomy
  • Melanoma
  • Micrometastasis
  • RT-PCR
  • Sentinel lymph node

ASJC Scopus subject areas

  • Surgery

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