"rickettsia amblyommii" Induces cross protection against lethal rocky mountain spotted fever in a guinea pig model

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15 Citations (Scopus)

Abstract

Rocky Mountain spotted fever (RMSF) is a severe illness caused by Rickettsia rickettsii for which there is no available vaccine. We hypothesize that exposure to the highly prevalent, relatively nonpathogenic "Rickettsia amblyommii" protects against R. rickettsii challenge. To test this hypothesis, guinea pigs were inoculated with "R. amblyommii." After inoculation, the animals showed no signs of illness. When later challenged with lethal doses of R. rickettsii, those previously exposed to "R. amblyommii" remained well, whereas unimmunized controls developed severe illness and died. We conclude that "R. amblyommii" induces an immune response that protects from illness and death in the guinea pig model of RMSF. These results provide a basis for exploring the use of low-virulence rickettsiae as a platform to develop live attenuated vaccine candidates to prevent severe rickettsioses.

Original languageEnglish (US)
Pages (from-to)557-562
Number of pages6
JournalVector-Borne and Zoonotic Diseases
Volume14
Issue number8
DOIs
StatePublished - Aug 1 2014

Fingerprint

Rickettsia rickettsii
Rocky Mountain Spotted Fever
Cross Protection
Rickettsia
Guinea Pigs
Attenuated Vaccines
Virulence
Vaccines

Keywords

  • "Rickettsia amblyommii"
  • Cross protection
  • Rickettsia rickettsia
  • Rocky Mountain spotted fever

ASJC Scopus subject areas

  • Infectious Diseases
  • Microbiology
  • Virology

Cite this

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title = "{"}rickettsia amblyommii{"} Induces cross protection against lethal rocky mountain spotted fever in a guinea pig model",
abstract = "Rocky Mountain spotted fever (RMSF) is a severe illness caused by Rickettsia rickettsii for which there is no available vaccine. We hypothesize that exposure to the highly prevalent, relatively nonpathogenic {"}Rickettsia amblyommii{"} protects against R. rickettsii challenge. To test this hypothesis, guinea pigs were inoculated with {"}R. amblyommii.{"} After inoculation, the animals showed no signs of illness. When later challenged with lethal doses of R. rickettsii, those previously exposed to {"}R. amblyommii{"} remained well, whereas unimmunized controls developed severe illness and died. We conclude that {"}R. amblyommii{"} induces an immune response that protects from illness and death in the guinea pig model of RMSF. These results provide a basis for exploring the use of low-virulence rickettsiae as a platform to develop live attenuated vaccine candidates to prevent severe rickettsioses.",
keywords = "{"}Rickettsia amblyommii{"}, Cross protection, Rickettsia rickettsia, Rocky Mountain spotted fever",
author = "Lucas Blanton and Mendell, {Nicole L.} and David Walker and Donald Bouyer",
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T1 - "rickettsia amblyommii" Induces cross protection against lethal rocky mountain spotted fever in a guinea pig model

AU - Blanton, Lucas

AU - Mendell, Nicole L.

AU - Walker, David

AU - Bouyer, Donald

PY - 2014/8/1

Y1 - 2014/8/1

N2 - Rocky Mountain spotted fever (RMSF) is a severe illness caused by Rickettsia rickettsii for which there is no available vaccine. We hypothesize that exposure to the highly prevalent, relatively nonpathogenic "Rickettsia amblyommii" protects against R. rickettsii challenge. To test this hypothesis, guinea pigs were inoculated with "R. amblyommii." After inoculation, the animals showed no signs of illness. When later challenged with lethal doses of R. rickettsii, those previously exposed to "R. amblyommii" remained well, whereas unimmunized controls developed severe illness and died. We conclude that "R. amblyommii" induces an immune response that protects from illness and death in the guinea pig model of RMSF. These results provide a basis for exploring the use of low-virulence rickettsiae as a platform to develop live attenuated vaccine candidates to prevent severe rickettsioses.

AB - Rocky Mountain spotted fever (RMSF) is a severe illness caused by Rickettsia rickettsii for which there is no available vaccine. We hypothesize that exposure to the highly prevalent, relatively nonpathogenic "Rickettsia amblyommii" protects against R. rickettsii challenge. To test this hypothesis, guinea pigs were inoculated with "R. amblyommii." After inoculation, the animals showed no signs of illness. When later challenged with lethal doses of R. rickettsii, those previously exposed to "R. amblyommii" remained well, whereas unimmunized controls developed severe illness and died. We conclude that "R. amblyommii" induces an immune response that protects from illness and death in the guinea pig model of RMSF. These results provide a basis for exploring the use of low-virulence rickettsiae as a platform to develop live attenuated vaccine candidates to prevent severe rickettsioses.

KW - "Rickettsia amblyommii"

KW - Cross protection

KW - Rickettsia rickettsia

KW - Rocky Mountain spotted fever

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