Rickettsia australis activates inflammasome in human and murine macrophages

Claire Smalley, Jeremy Bechelli, Dedeke Rockx-Brouwer, Tais Saito, Sasha R. Azar, Nahed Ismail, David Walker, Rong Fang

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Rickettsiae actively escape from vacuoles and replicate free in the cytoplasm of host cells, where inflammasomes survey the invading pathogens. In the present study, we investigated the interactions of Rickettsia australis with the inflammasome in both mouse and human macrophages. R. australis induced a significant level of IL-1β secretion by human macrophages, which was significantly reduced upon treatment with an inhibitor of caspase-1 compared to untreated controls, suggesting caspase-1-dependent inflammasome activation. Rickettsia induced significant secretion of IL-1β and IL-18 in vitro by infected mouse bone marrow-derived macrophages (BMMs) as early as 8-12 h post infection (p.i.) in a dose-dependent manner. Secretion of these cytokines was accompanied by cleavage of caspase-1 and was completely abrogated in BMMs deficient in caspase-1/caspase-11 or apoptosis-associated speck-like protein containing a caspase activation and recruitment domain (ASC), suggesting that R. australis activate the ASC-dependent inflammasome. Interestingly, in response to the same quantity of rickettsiae, NLRP3-/- BMMs significantly reduced the secretion level of IL-1β compared to wild type (WT) controls, suggesting that NLRP3 inflammasome contributes to cytosolic recognition of R. australis in vitro. Rickettsial load in spleen, but not liver and lung, of R. australis-infected NLRP3-/- mice was significantly greater compared to WT mice. These data suggest that NLRP3 inflammasome plays a role in host control of bacteria in vivo in a tissue-specific manner. Taken together, our data, for the first time, illustrate the activation of ASC-dependent inflammasome by R. australis in macrophages in which NLRP3 is involved.

Original languageEnglish (US)
Article numbere0157231
JournalPLoS One
Volume11
Issue number6
DOIs
StatePublished - Jun 1 2016

Fingerprint

Rickettsia australis
Inflammasomes
Rickettsia
Macrophages
caspase-1
macrophages
mice
interleukin-1
Caspase 1
secretion
bone marrow
Interleukin-1
Bone
caspase-11
Chemical activation
Caspases
interleukin-18
caspases
Interleukin-18
Pathogens

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Rickettsia australis activates inflammasome in human and murine macrophages. / Smalley, Claire; Bechelli, Jeremy; Rockx-Brouwer, Dedeke; Saito, Tais; Azar, Sasha R.; Ismail, Nahed; Walker, David; Fang, Rong.

In: PLoS One, Vol. 11, No. 6, e0157231, 01.06.2016.

Research output: Contribution to journalArticle

Smalley C, Bechelli J, Rockx-Brouwer D, Saito T, Azar SR, Ismail N et al. Rickettsia australis activates inflammasome in human and murine macrophages. PLoS One. 2016 Jun 1;11(6). e0157231. https://doi.org/10.1371/journal.pone.0157231
Smalley, Claire ; Bechelli, Jeremy ; Rockx-Brouwer, Dedeke ; Saito, Tais ; Azar, Sasha R. ; Ismail, Nahed ; Walker, David ; Fang, Rong. / Rickettsia australis activates inflammasome in human and murine macrophages. In: PLoS One. 2016 ; Vol. 11, No. 6.
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