Rickettsia bellii and Rickettsia amblyommii in Amblyomma ticks from the State of Rondônia, Western Amazon, Brazil

Marcelo B. Labruna, Ted Whitworth, Donald Bouyer, Jere McBride, Luis Marcelo A Camargo, Erney P. Camargo, Vsevolod Popov, David Walker

Research output: Contribution to journalArticle

171 Citations (Scopus)

Abstract

This study evaluates the rickettsial presence in Amblyomma ticks from eight areas of the Amazon forest in Rondônia, Brazil. The following tick species (number in parentheses) were examined: Amblyomma ovale Koch (121), Amblyomma cajennense (F.) (41), Amblyomma naponense (Packard) (36), Amblyomma scalpturatum Neumann (35), Amblyomma oblongoguttatum Koch (30), Amblyomma incisum Neumann (27), Amblyomma rotundatum Koch (16), Amblyomma coelebs Neumann (10), and Amblyomma humerale Koch (6). Ticks were examined individually or in pools (2-10 ticks) by polymerase chain reaction (PCR) targeting the gltA gene. The PCR-determined minimal infection rate for each tick species was A. ovale 28%, A. cajennense 27%, A. naponense 0%, A. scalpturatum 11%, A. oblongoguttatum 3%, A. incisum 0%, A. rotundatum 87%, A. coelebs 10%, and A. humerale 50%. Partial sequences of the gltA gene of Rickettsia from A. ovale, A. scalpturatum, A. oblongoguttatum, A. rotundatum, and A. humerale were 99.9% (349/350) identical to Rickettsia bellii. DNA sequences of PCR products from A. cajennense and A. coelebs were 100% (350/350) identical to Rickettsia amblyommii. R. bellii organisms were isolated in Vero cells from A. scalpturatum, A. ovale, A. rotundatum, and A. oblongoguttatum, but only one of the isolates, cultured from A. scalpturatum, was established in continuous cell culture passage. R. amblyommii was isolated from A. cajennense and was successfully established in continuous passage in cell culture. R. amblyommii infection of Vero cells was analyzed by transmission electron microscopy. This study adds South America to the known geographic distribution of R. amblyommii and reports rickettsiae in six Amblyomma species for the first time.

Original languageEnglish (US)
Pages (from-to)1073-1081
Number of pages9
JournalJournal of Medical Entomology
Volume41
Issue number6
StatePublished - Nov 2004

Fingerprint

Rickettsia bellii
Rickettsia
Amblyomma
Ticks
Brazil
ticks
Amblyomma cajennense
Vero Cells
Polymerase Chain Reaction
Cell Culture Techniques
polymerase chain reaction
Gene Targeting
South America
DNA-Directed DNA Polymerase
Infection
Transmission Electron Microscopy
cell culture
nucleotide sequences
gene targeting
infection

Keywords

  • Amazon
  • Amblyomma
  • Brazil
  • Rickettsia
  • Ultrastructure

ASJC Scopus subject areas

  • Insect Science
  • veterinary(all)

Cite this

Rickettsia bellii and Rickettsia amblyommii in Amblyomma ticks from the State of Rondônia, Western Amazon, Brazil. / Labruna, Marcelo B.; Whitworth, Ted; Bouyer, Donald; McBride, Jere; Camargo, Luis Marcelo A; Camargo, Erney P.; Popov, Vsevolod; Walker, David.

In: Journal of Medical Entomology, Vol. 41, No. 6, 11.2004, p. 1073-1081.

Research output: Contribution to journalArticle

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