Rickettsia hoogstraalii sp. nov., isolated from hardand soft-bodied ticks

Darja Duh, Volga Punda-Polic, Tatjana Avsic-Zupanc, Donald Bouyer, David Walker, Vsevolod Popov, Mateja Jelovsek, Maja Gracner, Tomi Trilar, Nikola Bradaric, Timothy J. Kurtti, Jasna Strus

Research output: Contribution to journalArticle

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Abstract

A novel spotted fever group Rickettsia was found in Haemaphysalis sulcata ticks collected from sheep and goats in Croatia in 2006. At the same time, a genetically identical organism was coisolated with the embryonic cell line CCE3 obtained from the soft tick Carios capensis in Georgia, USA. In this study, further phenotypic and genotypic characteristics of the novel rickettsial strain present in H. sulcata ticks were investigated. Based on the cultivation of bacteria in mosquito and Vero cell cultures, the presence of rickettsiae in tick tissues and cell cultures [confirmed by transmission electron microscopy (TEM)] and the amplification and sequencing of five rickettsial genes, it was demonstrated that the novel Rickettsia strain fulfils the criteria to be classified as a novel species. The name Rickettsia hoogstraalii sp. nov. is proposed for the new strain. Rickettsia hoogstraalii sp. nov., an obligately intracellular bacterium, was grown in Vero cells and arthropod CCE3, ISE6 and C6/36 cell lines. The morphology of the cells of the novel species was typical of SFG rickettsiae. The small coccobacillary appearance of the bacteria was apparent with light microscopy. A Gram-negative bacterial cell wall and a cytoplasmic membrane separated by a narrow periplasmic space were visible by TEM. To date, Rickettsia hoogstraalii sp. nov. has been isolated from two species of ticks, H. sulcata and C. capensis. The novel species appears to be geographically widely distributed, having been detected in Croatia, Spain and Georgia, USA. Although no information is available regarding the possible pathogenicity of the novel species for vertebrate hosts, R. hoogstraalii sp. nov. has a cytopathic effect in Vero, CCE3 and ISE6 cells. Sequence analyses of the 16S rRNA, 17 kDa, gltA, ompA and ompB genes indicated that even though R. hoogstraalii sp. nov. was closely related to Rickettsia felis, it represents a separate species within the spotted fever group. The type strain of R. hoogstraalii sp. nov. is strain CroaticaT (=DSM 22243 T=UTMB 00003T).

Original languageEnglish (US)
Pages (from-to)977-984
Number of pages8
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume60
Issue number4
DOIs
StatePublished - Apr 2010

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Argasidae
Rickettsia
tick
ticks
Ticks
new species
Croatia
Vero Cells
bacterium
fever
transmission electron microscopy
Bacteria
Transmission Electron Microscopy
Carios
bacteria
cell culture
Rickettsia felis
cell lines
Fever
Cell Culture Techniques

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Rickettsia hoogstraalii sp. nov., isolated from hardand soft-bodied ticks. / Duh, Darja; Punda-Polic, Volga; Avsic-Zupanc, Tatjana; Bouyer, Donald; Walker, David; Popov, Vsevolod; Jelovsek, Mateja; Gracner, Maja; Trilar, Tomi; Bradaric, Nikola; Kurtti, Timothy J.; Strus, Jasna.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 60, No. 4, 04.2010, p. 977-984.

Research output: Contribution to journalArticle

Duh, D, Punda-Polic, V, Avsic-Zupanc, T, Bouyer, D, Walker, D, Popov, V, Jelovsek, M, Gracner, M, Trilar, T, Bradaric, N, Kurtti, TJ & Strus, J 2010, 'Rickettsia hoogstraalii sp. nov., isolated from hardand soft-bodied ticks', International Journal of Systematic and Evolutionary Microbiology, vol. 60, no. 4, pp. 977-984. https://doi.org/10.1099/ijs.0.011049-0
Duh, Darja ; Punda-Polic, Volga ; Avsic-Zupanc, Tatjana ; Bouyer, Donald ; Walker, David ; Popov, Vsevolod ; Jelovsek, Mateja ; Gracner, Maja ; Trilar, Tomi ; Bradaric, Nikola ; Kurtti, Timothy J. ; Strus, Jasna. / Rickettsia hoogstraalii sp. nov., isolated from hardand soft-bodied ticks. In: International Journal of Systematic and Evolutionary Microbiology. 2010 ; Vol. 60, No. 4. pp. 977-984.
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