Rickettsia monteiroi sp. nov., infecting the tick Amblyomma incisum in Brazil

Richard C. Pacheco, Jonas Moraes-Filho, Arlei Marcili, Leonardo J. Richtzenhain, Matias P J Szabó, Márcia H B Catroxo, Donald Bouyer, Marcelo B. Labruna

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Free-living adult Amblyomma incisum ticks were collected in an Atlantic rainforest area at Intervales State Park, State of São Paulo, Brazil. From an A. incisum specimen, rickettsiae were successfully isolated in Vero cell culture by the shell vial technique. Rickettsial isolation was confirmed by optical microscopy, transmission electron microscopy, and PCRs targeting portions of the rickettsial genes gltA, htrA, rrs, and sca1 on infected cells. Fragments of 1,089, 457, 1,362, and 443 nucleotides of the gltA, htrA, rrs, and sca1 genes, respectively, were sequenced. By BLAST analysis, the partial sequence of rrs of the A. incisum rickettsial isolate was closest to the corresponding sequence of Rickettsia bellii (99.1% similarity). The gltA partial sequence was closest to the corresponding sequences of "Candidatus Rickettsia tarasevichiae" (96.1% similarity) and Rickettsia canadensis (95.8% similarity). The htrA partial sequence was closest to the corresponding sequence of R. canadensis (89.8% similarity). The sca1 partial sequence was closest to the corresponding sequence of R. canadensis (95.2% similarity). Since our rickettsial isolate was genetically distinct from other Rickettsia species, we propose a new species designated Rickettsia monteiroi sp. nov. Phylogenetic analyses indicated that R. monteiroi belongs to the canadensis group within the genus Rickettsia, together with the species R. canadensis and "Candidatus R. tarasevichiae". Little or no antibody cross-reaction was observed between sera of R. monteiroi-inoculated guinea pigs and R. bellii-, Rickettsia rickettsii-, or R. canadensis-inoculated guinea pigs.

Original languageEnglish (US)
Pages (from-to)5207-5211
Number of pages5
JournalApplied and Environmental Microbiology
Volume77
Issue number15
DOIs
StatePublished - Aug 2011

Fingerprint

Rickettsia canadensis
Rickettsia
Amblyomma
tick
Ticks
pig
Brazil
ticks
Rickettsia bellii
gene
new species
rainforest
targeting
antibody
transmission electron microscopy
microscopy
serum
guinea pigs
shell
phylogenetics

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Food Science
  • Biotechnology
  • Ecology

Cite this

Pacheco, R. C., Moraes-Filho, J., Marcili, A., Richtzenhain, L. J., Szabó, M. P. J., Catroxo, M. H. B., ... Labruna, M. B. (2011). Rickettsia monteiroi sp. nov., infecting the tick Amblyomma incisum in Brazil. Applied and Environmental Microbiology, 77(15), 5207-5211. https://doi.org/10.1128/AEM.05166-11

Rickettsia monteiroi sp. nov., infecting the tick Amblyomma incisum in Brazil. / Pacheco, Richard C.; Moraes-Filho, Jonas; Marcili, Arlei; Richtzenhain, Leonardo J.; Szabó, Matias P J; Catroxo, Márcia H B; Bouyer, Donald; Labruna, Marcelo B.

In: Applied and Environmental Microbiology, Vol. 77, No. 15, 08.2011, p. 5207-5211.

Research output: Contribution to journalArticle

Pacheco, RC, Moraes-Filho, J, Marcili, A, Richtzenhain, LJ, Szabó, MPJ, Catroxo, MHB, Bouyer, D & Labruna, MB 2011, 'Rickettsia monteiroi sp. nov., infecting the tick Amblyomma incisum in Brazil', Applied and Environmental Microbiology, vol. 77, no. 15, pp. 5207-5211. https://doi.org/10.1128/AEM.05166-11
Pacheco RC, Moraes-Filho J, Marcili A, Richtzenhain LJ, Szabó MPJ, Catroxo MHB et al. Rickettsia monteiroi sp. nov., infecting the tick Amblyomma incisum in Brazil. Applied and Environmental Microbiology. 2011 Aug;77(15):5207-5211. https://doi.org/10.1128/AEM.05166-11
Pacheco, Richard C. ; Moraes-Filho, Jonas ; Marcili, Arlei ; Richtzenhain, Leonardo J. ; Szabó, Matias P J ; Catroxo, Márcia H B ; Bouyer, Donald ; Labruna, Marcelo B. / Rickettsia monteiroi sp. nov., infecting the tick Amblyomma incisum in Brazil. In: Applied and Environmental Microbiology. 2011 ; Vol. 77, No. 15. pp. 5207-5211.
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