Rift Valley fever virus NSs mRNA is transcribed from an incoming anti-viral-sense S RNA segment

Tetsuro Ikegami, Sungyong Won, C. J. Peters, Shinji Makino

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

Analysis of purified Rift Valley fever virus (RVFV) particles demonstrated the presence of three negative-sense RNA genomes, plus three anti-viral-sense RNA segments. The virion-associated anti-viral-sense S segment served as a template for the synthesis of NSs mRNA immediately after infection. NSs protein synthesis also occurred early in infection, suggesting that NSs protein produced early in infection probably has biologically significant roles in virus replication and/or survival in the host. Translation inhibitor treatment of mammalian cells infected with viruses belonging to the Bunyaviridae family generally inhibits viral mRNA synthesis. However, RVFV NSs mRNA synthesis, but not N mRNA synthesis, was resistant to puromycin treatment during primary transcription, pointing to the uniqueness of RVFV NSs mRNA synthesis.

Original languageEnglish (US)
Pages (from-to)12106-12111
Number of pages6
JournalJournal of Virology
Volume79
Issue number18
DOIs
StatePublished - Sep 2005

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Rift Valley fever virus
RNA
Messenger RNA
synthesis
virion
Virion
Infection
Bunyaviridae
infection
puromycin
Antisense RNA
Puromycin
Viral RNA
Virus Replication
virus replication
translation (genetics)
Proteins
transcription (genetics)
protein synthesis
Genome

ASJC Scopus subject areas

  • Immunology

Cite this

Rift Valley fever virus NSs mRNA is transcribed from an incoming anti-viral-sense S RNA segment. / Ikegami, Tetsuro; Won, Sungyong; Peters, C. J.; Makino, Shinji.

In: Journal of Virology, Vol. 79, No. 18, 09.2005, p. 12106-12111.

Research output: Contribution to journalArticle

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