RIG-I enhanced interferon independent apoptosis upon Junin virus infection

Olga A. Kolokoltsova, Ashley M. Grant, Cheng Huang, Jennifer K. Smith, Allison L. Poussard, Bing Tian, Allan R. Brasier, Clarence J. Peters, Chien-Te Tseng, Juan C. De La Torre, Slobodan Paessler

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Junin virus (JUNV) is the etiological agent of Argentine hemorrhagic fever (AHF), a human disease with a high case-fatality rate. It is widely accepted that arenaviral infections, including JUNV infections, are generally non-cytopathic. In contrast, here we demonstrated apoptosis induction in human lung epithelial carcinoma (A549), human hepatocarcinoma and Vero cells upon infection with the attenuated Candid#1 strain of, JUNV as determined by phosphatidylserine (PS) translocation, Caspase 3 (CASP3) activation, Poly (ADP-ribose) polymerase (PARP) cleavage and/or chromosomal DNA fragmentation. Moreover, as determined by DNA fragmentation, we found that the pathogenic Romero strain of JUNV was less cytopathic than Candid#1 in human hepatocarcinoma and Vero, but more apoptotic in A549 and Vero E6 cells. Additionally, we found that JUNV-induced apoptosis was enhanced by RIG-I signaling. Consistent with the previously reported role of RIG-I like helicase (RLH) signaling in initiating programmed cell death, we showed that cell death or DNA fragmentation of Candid#1-infected A549 cells was decreased upon siRNA or shRNA silencing of components of RIG-I pathway in spite of increased virus production. Similarly, we observed decreased DNA fragmentation in JUNV-infected human hepatocarcinoma cells deficient for RIG-I when compared with that of RIG-I-competent cells. In addition, DNA fragmentation detected upon Candid#1 infection of type I interferon (IFN)-deficient Vero cells suggested a type I IFN-independent mechanism of apoptosis induction in response to JUNV. Our work demonstrated for the first time apoptosis induction in various cells of mammalian origin in response to JUNV infection and partial mechanism of this cell death.

Original languageEnglish (US)
Article numbere99610
JournalPLoS One
Volume9
Issue number6
DOIs
StatePublished - Jun 11 2014

Fingerprint

Junin virus
Virus Diseases
interferons
Viruses
Interferons
apoptosis
Apoptosis
DNA fragmentation
DNA Fragmentation
infection
Vero Cells
Cell death
Interferon Type I
DNA
cells
Cell Death
Small Interfering RNA
cell death
Infection
NAD ADP-ribosyltransferase

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

RIG-I enhanced interferon independent apoptosis upon Junin virus infection. / Kolokoltsova, Olga A.; Grant, Ashley M.; Huang, Cheng; Smith, Jennifer K.; Poussard, Allison L.; Tian, Bing; Brasier, Allan R.; Peters, Clarence J.; Tseng, Chien-Te; De La Torre, Juan C.; Paessler, Slobodan.

In: PLoS One, Vol. 9, No. 6, e99610, 11.06.2014.

Research output: Contribution to journalArticle

Kolokoltsova, OA, Grant, AM, Huang, C, Smith, JK, Poussard, AL, Tian, B, Brasier, AR, Peters, CJ, Tseng, C-T, De La Torre, JC & Paessler, S 2014, 'RIG-I enhanced interferon independent apoptosis upon Junin virus infection', PLoS One, vol. 9, no. 6, e99610. https://doi.org/10.1371/journal.pone.0099610
Kolokoltsova, Olga A. ; Grant, Ashley M. ; Huang, Cheng ; Smith, Jennifer K. ; Poussard, Allison L. ; Tian, Bing ; Brasier, Allan R. ; Peters, Clarence J. ; Tseng, Chien-Te ; De La Torre, Juan C. ; Paessler, Slobodan. / RIG-I enhanced interferon independent apoptosis upon Junin virus infection. In: PLoS One. 2014 ; Vol. 9, No. 6.
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abstract = "Junin virus (JUNV) is the etiological agent of Argentine hemorrhagic fever (AHF), a human disease with a high case-fatality rate. It is widely accepted that arenaviral infections, including JUNV infections, are generally non-cytopathic. In contrast, here we demonstrated apoptosis induction in human lung epithelial carcinoma (A549), human hepatocarcinoma and Vero cells upon infection with the attenuated Candid#1 strain of, JUNV as determined by phosphatidylserine (PS) translocation, Caspase 3 (CASP3) activation, Poly (ADP-ribose) polymerase (PARP) cleavage and/or chromosomal DNA fragmentation. Moreover, as determined by DNA fragmentation, we found that the pathogenic Romero strain of JUNV was less cytopathic than Candid#1 in human hepatocarcinoma and Vero, but more apoptotic in A549 and Vero E6 cells. Additionally, we found that JUNV-induced apoptosis was enhanced by RIG-I signaling. Consistent with the previously reported role of RIG-I like helicase (RLH) signaling in initiating programmed cell death, we showed that cell death or DNA fragmentation of Candid#1-infected A549 cells was decreased upon siRNA or shRNA silencing of components of RIG-I pathway in spite of increased virus production. Similarly, we observed decreased DNA fragmentation in JUNV-infected human hepatocarcinoma cells deficient for RIG-I when compared with that of RIG-I-competent cells. In addition, DNA fragmentation detected upon Candid#1 infection of type I interferon (IFN)-deficient Vero cells suggested a type I IFN-independent mechanism of apoptosis induction in response to JUNV. Our work demonstrated for the first time apoptosis induction in various cells of mammalian origin in response to JUNV infection and partial mechanism of this cell death.",
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AU - Brasier, Allan R.

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