RNA genome stability of Toscana virus during serial transovarial transmission in the sandfly Phlebotomus perniciosus

Pamuk A. Bilsel, Robert B. Tesh, Stuart T. Nichol

Research output: Contribution to journalArticle

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We have carried out a T1 ribonuclease fingerprinting analysis of the RNA genomes of Toscana virus isolates from successive generations of an experimentally virus-infected laboratory colony of Phlebotomus perniciosus sandflies. This analysis detected no virus RNA genome changes during transovarial transmission of the virus over 12 sandfly generations (a period of almost 2 years). These results demonstrate that although RNA viruses can exhibit high rates of mutational change under a variety of conditions, Toscana virus RNA genomes can be maintained in a stable manner during repeated transovarial virus transmission in the natural insect host. The implications of these results for insect RNA virus evolution are discussed.

Original languageEnglish (US)
Pages (from-to)87-94
Number of pages8
JournalVirus Research
Issue number1
StatePublished - Aug 1988



  • Insect model
  • Phlebovirus
  • RNA virus evolution
  • T1 RNase fingerprinting

ASJC Scopus subject areas

  • Cancer Research
  • Virology
  • Infectious Diseases

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