RNA oxidation is a prominent feature of vulnerable neurons in Alzheimer's disease

Akihiko Nunomura, George Perry, Miguel Pappolla, Ramon Wade, Keisuke Hirai, Shigeru Chiba, Mark A. Smith

Research output: Contribution to journalArticle

591 Citations (Scopus)

Abstract

In this study we used an in situ approach to identify the oxidized nucleosides 8-hydroxydeoxyguanosine (8OHdG) and 8-hydroxyguanosine (8OHG), markers of oxidative damage to DNA and RNA, respectively, in cases of Alzheimer's disease (AD). The goal was to determine whether nuclear and mitochondrial DNA as well as RNA is damaged in AD. Immunoreactivity with monoclonal antibodies 1F7 or 15A3 recognizing both 8OHdG and 8OHG was prominent in the cytoplasm and to a lesser extent in the nucleolus and nuclear envelope in neurons within the hippocampus, subiculum, and entorhinal cortex as well as frontal, temporal, and occipital neocortex in cases of AD, whereas similar structures were immunolabeled only faintly in controls. Relative density measurement showed that there was a significant increase (p < 0.0001) in 8OHdG and 8OHG immunoreactivity with 1F7 in cases of AD (n = 22) as compared with senile (n = 13), presenile (n = 10), or young controls (n = 4). Surprisingly, the oxidized nucleoside was associated predominantly with RNA because immunoreaction was diminished greatly by preincubation in RNase but only slightly by DNase. This is the first evidence of increased RNA oxidation restricted to vulnerable neurons in AD. The subcellular localization of damaged RNA showing cytoplasmic predominance is consistent with the hypothesis that mitochondria may be a major source of reactive oxygen species that cause oxidative damage in AD.

Original languageEnglish (US)
Pages (from-to)1959-1964
Number of pages6
JournalJournal of Neuroscience
Volume19
Issue number6
StatePublished - Mar 15 1999
Externally publishedYes

Fingerprint

Alzheimer Disease
RNA
Neurons
Nucleosides
Hippocampus
Entorhinal Cortex
Specific Gravity
Deoxyribonucleases
Neocortex
Nuclear Envelope
Ribonucleases
Mitochondrial DNA
DNA Damage
Reactive Oxygen Species
Mitochondria
Cytoplasm
Monoclonal Antibodies
8-hydroxyguanosine

Keywords

  • 8- hydroxyguanosine
  • 8-hydroxydeoxyguanosine
  • Alzheimer's disease
  • DNA oxidation
  • Mitochondrial damage
  • Oxidative stress
  • RNA oxidation

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Nunomura, A., Perry, G., Pappolla, M., Wade, R., Hirai, K., Chiba, S., & Smith, M. A. (1999). RNA oxidation is a prominent feature of vulnerable neurons in Alzheimer's disease. Journal of Neuroscience, 19(6), 1959-1964.

RNA oxidation is a prominent feature of vulnerable neurons in Alzheimer's disease. / Nunomura, Akihiko; Perry, George; Pappolla, Miguel; Wade, Ramon; Hirai, Keisuke; Chiba, Shigeru; Smith, Mark A.

In: Journal of Neuroscience, Vol. 19, No. 6, 15.03.1999, p. 1959-1964.

Research output: Contribution to journalArticle

Nunomura, A, Perry, G, Pappolla, M, Wade, R, Hirai, K, Chiba, S & Smith, MA 1999, 'RNA oxidation is a prominent feature of vulnerable neurons in Alzheimer's disease', Journal of Neuroscience, vol. 19, no. 6, pp. 1959-1964.
Nunomura A, Perry G, Pappolla M, Wade R, Hirai K, Chiba S et al. RNA oxidation is a prominent feature of vulnerable neurons in Alzheimer's disease. Journal of Neuroscience. 1999 Mar 15;19(6):1959-1964.
Nunomura, Akihiko ; Perry, George ; Pappolla, Miguel ; Wade, Ramon ; Hirai, Keisuke ; Chiba, Shigeru ; Smith, Mark A. / RNA oxidation is a prominent feature of vulnerable neurons in Alzheimer's disease. In: Journal of Neuroscience. 1999 ; Vol. 19, No. 6. pp. 1959-1964.
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