RNA recombination of coronavirus.

J. G. Keck, S. Makino, L. H. Soe, J. O. Fleming, S. A. Stohlman, M. M. Lai

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

We have previously shown that Mouse hepatitis virus (MHV) can undergo RNA-RNA recombination at a very high frequency (S. Makino, et al., J. Virol. 57, 729-737, 1986). To better define the mechanism of RNA recombination, we have performed additional crosses involving different MHV strains. We have obtained recombinant viruses with multiple cross-overs. The isolation of such recombinants further indicates the high frequency of coronavirus RNA recombination. By using cell fusion as a selection marker, we have also obtained recombinants between MHV-2 and A59 strains. Some of these recombinants have cross-overs in the 3'-end genes of the genome, thus demonstrating that recombination could occur along the entire genome. Finally, we have obtained recombinants by selecting with neutralizing monoclonal antibodies. These recombinants have cross-overs within gene C which encodes the peplomer protein. The genetic structure of these recombinants allowed us to determine the important domains of the peplomer proteins.

Original languageEnglish (US)
Pages (from-to)99-107
Number of pages9
JournalAdvances in experimental medicine and biology
Volume218
DOIs
StatePublished - 1987
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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