RNA recombination of coronavirus.

J. G. Keck, Shinji Makino, L. H. Soe, J. O. Fleming, S. A. Stohlman, M. M. Lai

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

We have previously shown that Mouse hepatitis virus (MHV) can undergo RNA-RNA recombination at a very high frequency (S. Makino, et al., J. Virol. 57, 729-737, 1986). To better define the mechanism of RNA recombination, we have performed additional crosses involving different MHV strains. We have obtained recombinant viruses with multiple cross-overs. The isolation of such recombinants further indicates the high frequency of coronavirus RNA recombination. By using cell fusion as a selection marker, we have also obtained recombinants between MHV-2 and A59 strains. Some of these recombinants have cross-overs in the 3'-end genes of the genome, thus demonstrating that recombination could occur along the entire genome. Finally, we have obtained recombinants by selecting with neutralizing monoclonal antibodies. These recombinants have cross-overs within gene C which encodes the peplomer protein. The genetic structure of these recombinants allowed us to determine the important domains of the peplomer proteins.

Original languageEnglish (US)
Pages (from-to)99-107
Number of pages9
JournalAdvances in Experimental Medicine and Biology
Volume218
StatePublished - 1987
Externally publishedYes

Fingerprint

Coronavirus
Murine hepatitis virus
Viruses
Genetic Recombination
Genes
RNA
Genome
Cell Fusion
Genetic Structures
Neutralizing Antibodies
Proteins
Fusion reactions
Monoclonal Antibodies

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Keck, J. G., Makino, S., Soe, L. H., Fleming, J. O., Stohlman, S. A., & Lai, M. M. (1987). RNA recombination of coronavirus. Advances in Experimental Medicine and Biology, 218, 99-107.

RNA recombination of coronavirus. / Keck, J. G.; Makino, Shinji; Soe, L. H.; Fleming, J. O.; Stohlman, S. A.; Lai, M. M.

In: Advances in Experimental Medicine and Biology, Vol. 218, 1987, p. 99-107.

Research output: Contribution to journalArticle

Keck, JG, Makino, S, Soe, LH, Fleming, JO, Stohlman, SA & Lai, MM 1987, 'RNA recombination of coronavirus.', Advances in Experimental Medicine and Biology, vol. 218, pp. 99-107.
Keck JG, Makino S, Soe LH, Fleming JO, Stohlman SA, Lai MM. RNA recombination of coronavirus. Advances in Experimental Medicine and Biology. 1987;218:99-107.
Keck, J. G. ; Makino, Shinji ; Soe, L. H. ; Fleming, J. O. ; Stohlman, S. A. ; Lai, M. M. / RNA recombination of coronavirus. In: Advances in Experimental Medicine and Biology. 1987 ; Vol. 218. pp. 99-107.
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