RNF151, a testis-specific RING finger protein, interacts with dysbindin

Hong Nian, Cuihong Fan, Shangying Liao, Yuqiang Shi, Keying Zhang, Yixun Liu, Chunsheng Han

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

RING finger proteins play important roles in spermatogenesis. Here, we report that a novel RING finger protein RNF151, with a C3HC4-type RING finger domain, a putative nuclear localization signal (NLS), and a TRAF-type zinc finger domain, was exclusively expressed in the mouse testis and developmentally regulated during spermatogenesis. While RNF151 mRNA was present in round spermatids, its protein was expressed in elongating spermatids of the stage VIII-IX seminiferous tubules. The NLS together with the RING domain were necessary and sufficient for the nuclear localization of RNF151-EGFP in transfected cells. Yeast two-hybrid screening identified the physical interaction of mouse RNF151 and dysbindin, which was confirmed by the co-immunoprecipitation of the proteins and by their co-localization in intact cells. As dysbindin has lately been shown to be involved in membrane biogenesis and fusion, a key process for acrosome formation, we propose that RNF151 may play a role in acrosome formation.

Original languageEnglish (US)
Pages (from-to)157-163
Number of pages7
JournalArchives of Biochemistry and Biophysics
Volume465
Issue number1
DOIs
StatePublished - Sep 1 2007
Externally publishedYes

Fingerprint

Testis
Nuclear Localization Signals
Acrosome
Spermatids
Spermatogenesis
Proteins
RING Finger Domains
Membrane Fusion
Seminiferous Tubules
Zinc Fingers
Immunoprecipitation
Yeast
Zinc
Screening
Fusion reactions
Yeasts
Membranes
Messenger RNA

Keywords

  • Dysbindin
  • RING finger protein
  • RNF151
  • Spermatids
  • Spermatogenesis
  • Testis

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

RNF151, a testis-specific RING finger protein, interacts with dysbindin. / Nian, Hong; Fan, Cuihong; Liao, Shangying; Shi, Yuqiang; Zhang, Keying; Liu, Yixun; Han, Chunsheng.

In: Archives of Biochemistry and Biophysics, Vol. 465, No. 1, 01.09.2007, p. 157-163.

Research output: Contribution to journalArticle

Nian, H, Fan, C, Liao, S, Shi, Y, Zhang, K, Liu, Y & Han, C 2007, 'RNF151, a testis-specific RING finger protein, interacts with dysbindin', Archives of Biochemistry and Biophysics, vol. 465, no. 1, pp. 157-163. https://doi.org/10.1016/j.abb.2007.05.013
Nian, Hong ; Fan, Cuihong ; Liao, Shangying ; Shi, Yuqiang ; Zhang, Keying ; Liu, Yixun ; Han, Chunsheng. / RNF151, a testis-specific RING finger protein, interacts with dysbindin. In: Archives of Biochemistry and Biophysics. 2007 ; Vol. 465, No. 1. pp. 157-163.
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