Abstract
Spinal cord injury (SCI) often results in abnormal pain syndromes in patients. We present a recently developed SCI mammalian model of chronic central pain in which the spinal cord is contused at T8 using the NYU impactor device (10-g rod, 2.0-mm diameter, 12.5-mm drop height), an injury which is characterized behaviorally as moderate. Recovery of locomotor function was assessed with an open field test and scored using the open field test scale (BBB scale). Somatosensory tests of paw withdrawal responses accompanied by supraspinal responses to both mechanical punctate (von Frey hairs) and nonpunctate (4 mm diameter blunt probe) as well as thermal (radiant heat) peripheral stimuli were performed. Comparisons at the level of the individual animal between precontusion and postcontusion responses indicated significant increases in reactions to low threshold punctate mechanical stimuli, non-punctate stimuli and thermal stimuli (p > 0.05). To demonstrate the validity of this model as a central pain model, gabapentin, an agent used clinically for central pain, was given i.p. at 10 or 30 mg/kg. Gabapentin treatment significantly and reversibly changed the responses, consistent with the attenuation of the abnormal sensory behavior, and the attenuated responses lasted for the duration of the drug effect (up to 6 h). These results support the use of the spinal contusion model in the study of chronic central pain after SCI.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1205-1217 |
| Number of pages | 13 |
| Journal | Journal of Neurotrauma |
| Volume | 17 |
| Issue number | 12 |
| State | Published - 2000 |
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Keywords
- Allodynia
- Behavior
- Chronic pain
- Pain
- Spinal cord injury
ASJC Scopus subject areas
- Clinical Neurology
- Neuroscience(all)
Cite this
Rodent model of chronic central pain after spinal cord contusion injury and effects of gabapentin. / Hulsebosch, C. E.; Xu, G. Y.; Perez-Polo, J. R.; Westlund, K. N.; Taylor, C. P.; McAdoo, D. J.
In: Journal of Neurotrauma, Vol. 17, No. 12, 2000, p. 1205-1217.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Rodent model of chronic central pain after spinal cord contusion injury and effects of gabapentin
AU - Hulsebosch, C. E.
AU - Xu, G. Y.
AU - Perez-Polo, J. R.
AU - Westlund, K. N.
AU - Taylor, C. P.
AU - McAdoo, D. J.
PY - 2000
Y1 - 2000
N2 - Spinal cord injury (SCI) often results in abnormal pain syndromes in patients. We present a recently developed SCI mammalian model of chronic central pain in which the spinal cord is contused at T8 using the NYU impactor device (10-g rod, 2.0-mm diameter, 12.5-mm drop height), an injury which is characterized behaviorally as moderate. Recovery of locomotor function was assessed with an open field test and scored using the open field test scale (BBB scale). Somatosensory tests of paw withdrawal responses accompanied by supraspinal responses to both mechanical punctate (von Frey hairs) and nonpunctate (4 mm diameter blunt probe) as well as thermal (radiant heat) peripheral stimuli were performed. Comparisons at the level of the individual animal between precontusion and postcontusion responses indicated significant increases in reactions to low threshold punctate mechanical stimuli, non-punctate stimuli and thermal stimuli (p > 0.05). To demonstrate the validity of this model as a central pain model, gabapentin, an agent used clinically for central pain, was given i.p. at 10 or 30 mg/kg. Gabapentin treatment significantly and reversibly changed the responses, consistent with the attenuation of the abnormal sensory behavior, and the attenuated responses lasted for the duration of the drug effect (up to 6 h). These results support the use of the spinal contusion model in the study of chronic central pain after SCI.
AB - Spinal cord injury (SCI) often results in abnormal pain syndromes in patients. We present a recently developed SCI mammalian model of chronic central pain in which the spinal cord is contused at T8 using the NYU impactor device (10-g rod, 2.0-mm diameter, 12.5-mm drop height), an injury which is characterized behaviorally as moderate. Recovery of locomotor function was assessed with an open field test and scored using the open field test scale (BBB scale). Somatosensory tests of paw withdrawal responses accompanied by supraspinal responses to both mechanical punctate (von Frey hairs) and nonpunctate (4 mm diameter blunt probe) as well as thermal (radiant heat) peripheral stimuli were performed. Comparisons at the level of the individual animal between precontusion and postcontusion responses indicated significant increases in reactions to low threshold punctate mechanical stimuli, non-punctate stimuli and thermal stimuli (p > 0.05). To demonstrate the validity of this model as a central pain model, gabapentin, an agent used clinically for central pain, was given i.p. at 10 or 30 mg/kg. Gabapentin treatment significantly and reversibly changed the responses, consistent with the attenuation of the abnormal sensory behavior, and the attenuated responses lasted for the duration of the drug effect (up to 6 h). These results support the use of the spinal contusion model in the study of chronic central pain after SCI.
KW - Allodynia
KW - Behavior
KW - Chronic pain
KW - Pain
KW - Spinal cord injury
UR - http://www.scopus.com/inward/record.url?scp=0033673209&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033673209&partnerID=8YFLogxK
M3 - Article
C2 - 11186233
AN - SCOPUS:0033673209
VL - 17
SP - 1205
EP - 1217
JO - Journal of Neurotrauma
JF - Journal of Neurotrauma
SN - 0897-7151
IS - 12
ER -