Role of anti-L-selectin antibody in burn and smoke inhalation injury in sheep

Jiro Katahira, Kazunori Murakami, Frank C. Schmalstieg, Robert Cox, Hal Hawkins, Lillian D. Traber, Daniel L. Traber

Research output: Contribution to journalArticlepeer-review

19 Scopus citations


We hypothesized that the antibody neutralization of L-selectin would decrease the pulmonary abnormalities characteristic of burn and smoke inhalation injury. Three groups of sheep (n = 18) were prepared and randomized: the LAM-(1-3) group (n = 6) was injected intravenously with 1 mg/kg of leukocyte adhesion molecule (LAM)-(1-3) (mouse monoclonal antibody against L-selectin) 1 h after the injury, the control group (n = 6) was not injured or treated, and the nontreatment group (n = 6) was injured but not treated. All animals were mechanically ventilated during the 48-h experimental period. The ratio of arterial Po2 to inspired O2 fraction decreased in the LAM-(1-3) and nontreatment groups. Lung lymph flow and pulmonary microvascular permeability were elevated after injury. This elevation was significantly reduced when LAM-(1-3) was administered 1 h after injury. Nitrate/nitrite (NOx) amounts in plasma and lung lymph increased significantly after the combined injury. These changes were attenuated by posttreatment with LAM-(1-3). These results suggest that the changes in pulmonary transvascular fluid flux result from injury of lung endothelium by polymorphonuclear leukocytes. In conclusion, posttreatment with the antibody for L-selectin improved lung lymph flow and permeability index. L-selectin appears to be principally involved in the increased pulmonary transvascular fluid flux observed with burn/smoke insult. L-selectin may be a useful target in the treatment of acute lung injury after burn and smoke inhalation.

Original languageEnglish (US)
Pages (from-to)L1043-L1050
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Issue number5 27-5
StatePublished - Nov 1 2002
Externally publishedYes


  • Leukocyte adhesion molecule
  • Polymorphonuclear leukocyte
  • Thermal burn

ASJC Scopus subject areas

  • Physiology
  • Pulmonary and Respiratory Medicine
  • Physiology (medical)
  • Cell Biology


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