Role of BC loop residues in structure, function and antigenicity of the West Nile virus envelope protein receptor-binding domain III

Shuliu Zhang, Evgeniy I. Bovshik, Rodrigo Maillard, Gregory D. Gromowski, David E. Volk, Catherine H. Schein, Claire Y H Huang, David G. Gorenstein, James Lee, Alan Barrett, David Beasley

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Site-directed mutagenesis of residues in the BC loop (residues 329-333) of the envelope (E) protein domain III in a West Nile virus (WNV) infectious clone and in plasmids encoding recombinant WNV and dengue type 2 virus domain III proteins demonstrated a critical role for residues in this loop in the function and antigenicity of the E protein. This included a strict requirement for the tyrosine at residue 329 of WNV for virus viability and E domain III folding. The absence of an equivalent residue in this region of yellow fever group viruses and most tick-borne flavivirus suggests there is an evolutionary divergence in the molecular mechanisms of domain III folding employed by different flaviviruses.

Original languageEnglish (US)
Pages (from-to)85-91
Number of pages7
JournalVirology
Volume403
Issue number1
DOIs
StatePublished - Jul 2010

Fingerprint

Viral Envelope Proteins
West Nile virus
Protein Binding
Flavivirus
Yellow fever virus
Microbial Viability
Dengue Virus
Ticks
Site-Directed Mutagenesis
Tyrosine
Plasmids
Clone Cells
Proteins
Protein Domains

Keywords

  • Attenuation
  • Envelope protein
  • Flavivirus
  • Neutralization
  • Receptor binding domain
  • West Nile virus

ASJC Scopus subject areas

  • Virology

Cite this

Role of BC loop residues in structure, function and antigenicity of the West Nile virus envelope protein receptor-binding domain III. / Zhang, Shuliu; Bovshik, Evgeniy I.; Maillard, Rodrigo; Gromowski, Gregory D.; Volk, David E.; Schein, Catherine H.; Huang, Claire Y H; Gorenstein, David G.; Lee, James; Barrett, Alan; Beasley, David.

In: Virology, Vol. 403, No. 1, 07.2010, p. 85-91.

Research output: Contribution to journalArticle

Zhang, S, Bovshik, EI, Maillard, R, Gromowski, GD, Volk, DE, Schein, CH, Huang, CYH, Gorenstein, DG, Lee, J, Barrett, A & Beasley, D 2010, 'Role of BC loop residues in structure, function and antigenicity of the West Nile virus envelope protein receptor-binding domain III', Virology, vol. 403, no. 1, pp. 85-91. https://doi.org/10.1016/j.virol.2010.03.038
Zhang, Shuliu ; Bovshik, Evgeniy I. ; Maillard, Rodrigo ; Gromowski, Gregory D. ; Volk, David E. ; Schein, Catherine H. ; Huang, Claire Y H ; Gorenstein, David G. ; Lee, James ; Barrett, Alan ; Beasley, David. / Role of BC loop residues in structure, function and antigenicity of the West Nile virus envelope protein receptor-binding domain III. In: Virology. 2010 ; Vol. 403, No. 1. pp. 85-91.
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AU - Volk, David E.

AU - Schein, Catherine H.

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