Role of CNP in human airways: cGMP-mediated stimulation of ciliary beat frequency

C. A. Geary, C. W. Davis, A. M. Paradiso, R. C. Boucher

Research output: Contribution to journalArticlepeer-review

72 Scopus citations


Ciliated airway epithelial cells contribute to mucociliary transport systems via ciliary beating and electrolyte transport mechanisms. Both of these activities are regulated by agonists acting through intracellular calcium- and adenosine 3',5'-cyclic monophosphate (cAMP)-dependent processes (5, 15, 18, 27). This study examines the role of guanosine 3',5'-cyclic monophosphate (cGMP) in the regulation of both ciliary beat frequency (CBF) and electrolyte transport in human airway epithelia (HAE). In a previous report, cGMP production in HAE was observed after stimulation with either C- type natriuretic peptide (CNP) or sodium nitroprusside (SNP) (6). In this study, CNP was found to increase CBF by 30 ± 6.9%, and this effect was mimicked by the cGMP analogue, 8-bromoguanosine 3',5'-cyclic monophosphate (8-BrcGMP), but not by sodium nitroprusside. CNP-induced increases in CBF do not appear to be mediated by changes in either intracellular calcium or cAMP levels. Using modified Ussing chambers, we also investigated CNP's potential modulation of sodium and chloride transport rates. Neither CNP, nor SNP, nor 8-BrcGMP altered active ion transport rates. We conclude that CNP regulates ciliary beat via cGMP-dependent mechanisms, whereas no effect of CNP or cGMP on ion transport was detected.

Original languageEnglish (US)
Pages (from-to)L1021-L1028
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Issue number6 12-6
StatePublished - 1995
Externally publishedYes


  • C-type natriuretic peptide
  • adenosine 3',5'-cyclic monophosphate
  • calcium
  • guanylate cyclase
  • nitric oxide

ASJC Scopus subject areas

  • Physiology
  • Pulmonary and Respiratory Medicine
  • Physiology (medical)
  • Cell Biology


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