Role of CNP in human airways: cGMP-mediated stimulation of ciliary beat frequency

Cara Geary, C. W. Davis, A. M. Paradiso, R. C. Boucher

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

Ciliated airway epithelial cells contribute to mucociliary transport systems via ciliary beating and electrolyte transport mechanisms. Both of these activities are regulated by agonists acting through intracellular calcium- and adenosine 3',5'-cyclic monophosphate (cAMP)-dependent processes (5, 15, 18, 27). This study examines the role of guanosine 3',5'-cyclic monophosphate (cGMP) in the regulation of both ciliary beat frequency (CBF) and electrolyte transport in human airway epithelia (HAE). In a previous report, cGMP production in HAE was observed after stimulation with either C- type natriuretic peptide (CNP) or sodium nitroprusside (SNP) (6). In this study, CNP was found to increase CBF by 30 ± 6.9%, and this effect was mimicked by the cGMP analogue, 8-bromoguanosine 3',5'-cyclic monophosphate (8-BrcGMP), but not by sodium nitroprusside. CNP-induced increases in CBF do not appear to be mediated by changes in either intracellular calcium or cAMP levels. Using modified Ussing chambers, we also investigated CNP's potential modulation of sodium and chloride transport rates. Neither CNP, nor SNP, nor 8-BrcGMP altered active ion transport rates. We conclude that CNP regulates ciliary beat via cGMP-dependent mechanisms, whereas no effect of CNP or cGMP on ion transport was detected.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume268
Issue number6 12-6
StatePublished - 1995
Externally publishedYes

Fingerprint

C-Type Natriuretic Peptide
natriuretic peptides
Nitroprusside
Ion Transport
cyclic AMP
ion transport
Calcium
calcium
Cyclic AMP
Electrolytes
electrolytes
epithelium
Epithelium
Mucociliary Clearance
guanosine
Cyclic GMP
Sodium Chloride
agonists
chlorides
epithelial cells

Keywords

  • adenosine 3',5'-cyclic monophosphate
  • C-type natriuretic peptide
  • calcium
  • guanylate cyclase
  • nitric oxide

ASJC Scopus subject areas

  • Cell Biology
  • Physiology
  • Pulmonary and Respiratory Medicine
  • Agricultural and Biological Sciences(all)

Cite this

Role of CNP in human airways : cGMP-mediated stimulation of ciliary beat frequency. / Geary, Cara; Davis, C. W.; Paradiso, A. M.; Boucher, R. C.

In: American Journal of Physiology - Lung Cellular and Molecular Physiology, Vol. 268, No. 6 12-6, 1995.

Research output: Contribution to journalArticle

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