Role of CREB in vasoactive intestinal peptide-mediated wound healing in human bronchial epithelial cells

Cha Xiang Guan, Yan Ru Cui, Guo Ying Sun, Fang Yu, Chun Yan Tang, Yun Chao Li, Hui Jun Liu, Xiang Fang

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Vasoactive intestinal peptide (VIP) is one of the most important sensory neuropeptides in respiratory system. We previously reported that VIP enhances wound healing and proliferation of human bronchial epithelial cells (HBECs), and these effects are mediated by intracellular signaling molecules such as protein kinase A (PKA), protein kinase C (PKC), Calmodulin (CaM), and extracellular signal-regulated kinase (ERK). In the present study, we further investigated the role of cAMP Response Element Binding Protein (CREB) in VIP-promoted protective effects in mechanical-damaged HBECs. VIP-mediated wound healing and cell proliferation in HBECs was inhibited by CREB antisense oligonucleotides (ASO) in a time-dependent manner. VIP increased the CREB DNA binding activity and expression of the p-CREB that were inhibited by VIP receptor antagonist. Increased CREB DNA binding activity and expression of the p-CREB were also partially inhibited by PKA and ERK inhibitors. These results suggest that the VIP-mediated wound repair of HBECs is associated with activation of CREB via PKA and ERK dependent pathway. Crown

Original languageEnglish (US)
Pages (from-to)64-69
Number of pages6
JournalRegulatory Peptides
Volume153
Issue number1-3
DOIs
StatePublished - Feb 25 2009

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Keywords

  • DNA binding activity
  • Migration
  • p-CREB
  • Proliferation
  • Signal transduction

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Endocrinology
  • Physiology
  • Cellular and Molecular Neuroscience

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