Role of dietary antioxidants in human metapneumovirus infection

Narayana Komaravelli, John P. Kelley, Matteo P. Garofalo, Haotian Wu, Antonella Casola, Deepthi Kolli

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Human metapneumovirus (hMPV) is a major cause of respiratory tract infections in children, elderly and immunocompromised hosts, for which no vaccine or treatment are currently available. Oxidative stress and inflammatory responses represent important pathogenic mechanism(s) of hMPV infection. Here, we explored the potential protective role of dietary antioxidants in hMPV infection. Treatment of airway epithelial cells with resveratrol and quercetin during hMPV infection significantly reduced cellular oxidative damage, inflammatory mediator secretion and viral replication, without affecting viral gene transcription and protein synthesis, indicating that inhibition of viral replication occurred at the level of viral assembly and/or release. Modulation of proinflammatory mediator expression occurred through the inhibition of transcription factor nuclear factor (NF)-κB and interferon regulatory factor (IRF)-3 binding to their cognate site of endogenous gene promoters. Our results indicate the use of dietary antioxidants as an effective treatment approach for modulating hMPV induced lung oxidative damage and inflammation.

Original languageEnglish (US)
Pages (from-to)19-23
Number of pages5
JournalVirus Research
Volume200
Issue numberC
DOIs
StatePublished - Jan 1 2015

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Keywords

  • Human metapneumovirus
  • Quercetin
  • Resveratrol

ASJC Scopus subject areas

  • Cancer Research
  • Virology
  • Infectious Diseases

Cite this

Komaravelli, N., Kelley, J. P., Garofalo, M. P., Wu, H., Casola, A., & Kolli, D. (2015). Role of dietary antioxidants in human metapneumovirus infection. Virus Research, 200(C), 19-23. https://doi.org/10.1016/j.virusres.2015.01.018