TY - JOUR
T1 - Role of ENPP1 on adipocyte maturation
AU - Liang, Jian
AU - Fu, Mingui
AU - Ciociola, Ester
AU - Chandalia, Manisha
AU - Abate, Nicola
N1 - Copyright:
Copyright 2009 Elsevier B.V., All rights reserved.
PY - 2007/9/12
Y1 - 2007/9/12
N2 - Background. It is recognized that the ability of adipose tissue to expand in response to energy excess, i.e. adipocyte maturation, is important in determining systemic abnormalities in glucose and lipid metabolism. Ectonucleotide pyrophosphatase phosphodiesterase 1 (ENPP1, also known as PC-1) has been recently reported to be involved in the pathogenesis of insulin resistance and related.However, its role on adipose tissue physiology as a mechanism of systemic insulin resistance is not understood. This study was performed to evaluate whether ENPP1 is regulated during adipogenesis and whether over-expression in adipocytes can affect adipocyte maturation, a potential novel mechanism of ENPP1-related insulin resistance. Methodology/Principal Findings. ENPP1 expression was found down-requlated during 3T3-L1 maturation, and over-expression of human ENPP1 in 3T3-L1 (pQCXIP-ENPP1 vector) resulted in adipocyte insulin resistance and in defective adipocyte maturation. Adipocyte maturation was more efficient in mesenchymal embryonal cells from ENPP1 knockout mice than from wild-type. Conclusions. We identity ENPP1 as a novel mechanism of defective adipocyte maturation. This mechanism could contribute to the pathogenesis of insulin resistance in absence of obesity.
AB - Background. It is recognized that the ability of adipose tissue to expand in response to energy excess, i.e. adipocyte maturation, is important in determining systemic abnormalities in glucose and lipid metabolism. Ectonucleotide pyrophosphatase phosphodiesterase 1 (ENPP1, also known as PC-1) has been recently reported to be involved in the pathogenesis of insulin resistance and related.However, its role on adipose tissue physiology as a mechanism of systemic insulin resistance is not understood. This study was performed to evaluate whether ENPP1 is regulated during adipogenesis and whether over-expression in adipocytes can affect adipocyte maturation, a potential novel mechanism of ENPP1-related insulin resistance. Methodology/Principal Findings. ENPP1 expression was found down-requlated during 3T3-L1 maturation, and over-expression of human ENPP1 in 3T3-L1 (pQCXIP-ENPP1 vector) resulted in adipocyte insulin resistance and in defective adipocyte maturation. Adipocyte maturation was more efficient in mesenchymal embryonal cells from ENPP1 knockout mice than from wild-type. Conclusions. We identity ENPP1 as a novel mechanism of defective adipocyte maturation. This mechanism could contribute to the pathogenesis of insulin resistance in absence of obesity.
UR - http://www.scopus.com/inward/record.url?scp=41149171157&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=41149171157&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0000882
DO - 10.1371/journal.pone.0000882
M3 - Article
C2 - 17849011
AN - SCOPUS:41149171157
VL - 2
JO - PLoS One
JF - PLoS One
SN - 1932-6203
IS - 9
M1 - e882
ER -