Role of ENPP1 on adipocyte maturation

Jian Liang, Mingui Fu, Ester Ciociola, Manisha Chandalia, Nicola Abate

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Background. It is recognized that the ability of adipose tissue to expand in response to energy excess, i.e. adipocyte maturation, is important in determining systemic abnormalities in glucose and lipid metabolism. Ectonucleotide pyrophosphatase phosphodiesterase 1 (ENPP1, also known as PC-1) has been recently reported to be involved in the pathogenesis of insulin resistance and related.However, its role on adipose tissue physiology as a mechanism of systemic insulin resistance is not understood. This study was performed to evaluate whether ENPP1 is regulated during adipogenesis and whether over-expression in adipocytes can affect adipocyte maturation, a potential novel mechanism of ENPP1-related insulin resistance. Methodology/Principal Findings. ENPP1 expression was found down-requlated during 3T3-L1 maturation, and over-expression of human ENPP1 in 3T3-L1 (pQCXIP-ENPP1 vector) resulted in adipocyte insulin resistance and in defective adipocyte maturation. Adipocyte maturation was more efficient in mesenchymal embryonal cells from ENPP1 knockout mice than from wild-type. Conclusions. We identity ENPP1 as a novel mechanism of defective adipocyte maturation. This mechanism could contribute to the pathogenesis of insulin resistance in absence of obesity.

Original languageEnglish (US)
Article numbere882
JournalPLoS One
Volume2
Issue number9
DOIs
StatePublished - Sep 12 2007
Externally publishedYes

Fingerprint

adipocytes
Adipocytes
Insulin
insulin resistance
Insulin Resistance
Tissue
adipose tissue
Adipose Tissue
pathogenesis
Physiology
pyrophosphatases
Adipogenesis
Lipid Metabolism
Knockout Mice
lipid metabolism
Glucose
obesity
physiology
Obesity
glucose

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Liang, J., Fu, M., Ciociola, E., Chandalia, M., & Abate, N. (2007). Role of ENPP1 on adipocyte maturation. PLoS One, 2(9), [e882]. https://doi.org/10.1371/journal.pone.0000882

Role of ENPP1 on adipocyte maturation. / Liang, Jian; Fu, Mingui; Ciociola, Ester; Chandalia, Manisha; Abate, Nicola.

In: PLoS One, Vol. 2, No. 9, e882, 12.09.2007.

Research output: Contribution to journalArticle

Liang, J, Fu, M, Ciociola, E, Chandalia, M & Abate, N 2007, 'Role of ENPP1 on adipocyte maturation', PLoS One, vol. 2, no. 9, e882. https://doi.org/10.1371/journal.pone.0000882
Liang J, Fu M, Ciociola E, Chandalia M, Abate N. Role of ENPP1 on adipocyte maturation. PLoS One. 2007 Sep 12;2(9). e882. https://doi.org/10.1371/journal.pone.0000882
Liang, Jian ; Fu, Mingui ; Ciociola, Ester ; Chandalia, Manisha ; Abate, Nicola. / Role of ENPP1 on adipocyte maturation. In: PLoS One. 2007 ; Vol. 2, No. 9.
@article{bc38452d0ef4427e8296ae662d7dc712,
title = "Role of ENPP1 on adipocyte maturation",
abstract = "Background. It is recognized that the ability of adipose tissue to expand in response to energy excess, i.e. adipocyte maturation, is important in determining systemic abnormalities in glucose and lipid metabolism. Ectonucleotide pyrophosphatase phosphodiesterase 1 (ENPP1, also known as PC-1) has been recently reported to be involved in the pathogenesis of insulin resistance and related.However, its role on adipose tissue physiology as a mechanism of systemic insulin resistance is not understood. This study was performed to evaluate whether ENPP1 is regulated during adipogenesis and whether over-expression in adipocytes can affect adipocyte maturation, a potential novel mechanism of ENPP1-related insulin resistance. Methodology/Principal Findings. ENPP1 expression was found down-requlated during 3T3-L1 maturation, and over-expression of human ENPP1 in 3T3-L1 (pQCXIP-ENPP1 vector) resulted in adipocyte insulin resistance and in defective adipocyte maturation. Adipocyte maturation was more efficient in mesenchymal embryonal cells from ENPP1 knockout mice than from wild-type. Conclusions. We identity ENPP1 as a novel mechanism of defective adipocyte maturation. This mechanism could contribute to the pathogenesis of insulin resistance in absence of obesity.",
author = "Jian Liang and Mingui Fu and Ester Ciociola and Manisha Chandalia and Nicola Abate",
year = "2007",
month = "9",
day = "12",
doi = "10.1371/journal.pone.0000882",
language = "English (US)",
volume = "2",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "9",

}

TY - JOUR

T1 - Role of ENPP1 on adipocyte maturation

AU - Liang, Jian

AU - Fu, Mingui

AU - Ciociola, Ester

AU - Chandalia, Manisha

AU - Abate, Nicola

PY - 2007/9/12

Y1 - 2007/9/12

N2 - Background. It is recognized that the ability of adipose tissue to expand in response to energy excess, i.e. adipocyte maturation, is important in determining systemic abnormalities in glucose and lipid metabolism. Ectonucleotide pyrophosphatase phosphodiesterase 1 (ENPP1, also known as PC-1) has been recently reported to be involved in the pathogenesis of insulin resistance and related.However, its role on adipose tissue physiology as a mechanism of systemic insulin resistance is not understood. This study was performed to evaluate whether ENPP1 is regulated during adipogenesis and whether over-expression in adipocytes can affect adipocyte maturation, a potential novel mechanism of ENPP1-related insulin resistance. Methodology/Principal Findings. ENPP1 expression was found down-requlated during 3T3-L1 maturation, and over-expression of human ENPP1 in 3T3-L1 (pQCXIP-ENPP1 vector) resulted in adipocyte insulin resistance and in defective adipocyte maturation. Adipocyte maturation was more efficient in mesenchymal embryonal cells from ENPP1 knockout mice than from wild-type. Conclusions. We identity ENPP1 as a novel mechanism of defective adipocyte maturation. This mechanism could contribute to the pathogenesis of insulin resistance in absence of obesity.

AB - Background. It is recognized that the ability of adipose tissue to expand in response to energy excess, i.e. adipocyte maturation, is important in determining systemic abnormalities in glucose and lipid metabolism. Ectonucleotide pyrophosphatase phosphodiesterase 1 (ENPP1, also known as PC-1) has been recently reported to be involved in the pathogenesis of insulin resistance and related.However, its role on adipose tissue physiology as a mechanism of systemic insulin resistance is not understood. This study was performed to evaluate whether ENPP1 is regulated during adipogenesis and whether over-expression in adipocytes can affect adipocyte maturation, a potential novel mechanism of ENPP1-related insulin resistance. Methodology/Principal Findings. ENPP1 expression was found down-requlated during 3T3-L1 maturation, and over-expression of human ENPP1 in 3T3-L1 (pQCXIP-ENPP1 vector) resulted in adipocyte insulin resistance and in defective adipocyte maturation. Adipocyte maturation was more efficient in mesenchymal embryonal cells from ENPP1 knockout mice than from wild-type. Conclusions. We identity ENPP1 as a novel mechanism of defective adipocyte maturation. This mechanism could contribute to the pathogenesis of insulin resistance in absence of obesity.

UR - http://www.scopus.com/inward/record.url?scp=41149171157&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=41149171157&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0000882

DO - 10.1371/journal.pone.0000882

M3 - Article

C2 - 17849011

AN - SCOPUS:41149171157

VL - 2

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 9

M1 - e882

ER -