Role of gastric epithelial cell-derived transforming growth factor β in reduced CD4+ T cell proliferation and development of regulatory T cells during Helicobacter pylori infection

Ellen J. Beswick, Iryna Pinchuk, Rachel B. Earley, David A. Schmitt, Victor Reyes

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Gastric epithelial cells (GECs) express the class II major histocompatibility complex (MHC) and costimulatory molecules, enabling them to act as antigen-presenting cells (APCs) and affect local T cell responses. During Helicobacter pylori infection, GECs respond by releasing proinflammatory cytokines and by increasing the surface expression of immunologically relevant receptors, including class II MHC. The CD4+ T cell response during H. pylori infection is skewed toward a Th1 response, but these cells remain hyporesponsive. Activated T cells show decreased proliferation during H. pylori infection, and CD4+ CD25+ FoxP3+ regulatory T cells (Tregs) are present at the site of infection. In this study, we examined the mechanisms surrounding the CD4+ T cell responses during H. pylori infection and found that transforming growth factor β (TGF-β) plays a major role in these responses. GECs produced TGF-β1 and TGF-β2 in response to infection. Activated CD4+ T cells in culture with H. pylori-treated GECs were decreased in proliferation but increased upon neutralization of TGF-β Naïve CD4r+ T cell development into Tregs was also enhanced in the presence of GEC-derived TGF-β. Herein, we demonstrate a role for GEC-produced TGF-β in the inhibition of CD4+ T cell responses seen during H. pylori infection.

Original languageEnglish (US)
Pages (from-to)2737-2745
Number of pages9
JournalInfection and Immunity
Volume79
Issue number7
DOIs
StatePublished - Jul 2011

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Transforming Growth Factors
Helicobacter Infections
Regulatory T-Lymphocytes
Helicobacter pylori
Stomach
Epithelial Cells
Cell Proliferation
T-Lymphocytes
Major Histocompatibility Complex
Th1 Cells
Antigen-Presenting Cells
Infection
Cell Culture Techniques
Cytokines

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Parasitology
  • Infectious Diseases

Cite this

Role of gastric epithelial cell-derived transforming growth factor β in reduced CD4+ T cell proliferation and development of regulatory T cells during Helicobacter pylori infection. / Beswick, Ellen J.; Pinchuk, Iryna; Earley, Rachel B.; Schmitt, David A.; Reyes, Victor.

In: Infection and Immunity, Vol. 79, No. 7, 07.2011, p. 2737-2745.

Research output: Contribution to journalArticle

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